Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25870

Experiment: A22 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25850 and BWI76_RS25855 overlap by 17 nucleotidesBWI76_RS25855 and BWI76_RS25860 are separated by 74 nucleotidesBWI76_RS25860 and BWI76_RS25865 overlap by 60 nucleotidesBWI76_RS25865 and BWI76_RS25870 are separated by 98 nucleotidesBWI76_RS25870 and BWI76_RS25875 are separated by 70 nucleotides BWI76_RS25850: BWI76_RS25850 - prepilin peptidase, at 5,350,290 to 5,350,766 _RS25850 BWI76_RS25855: BWI76_RS25855 - bacterioferritin, at 5,350,750 to 5,351,226 _RS25855 BWI76_RS25860: BWI76_RS25860 - bacterioferritin-associated ferredoxin, at 5,351,301 to 5,351,495 _RS25860 BWI76_RS25865: BWI76_RS25865 - ferredoxin, at 5,351,436 to 5,351,570 _RS25865 BWI76_RS25870: BWI76_RS25870 - elongation factor Tu, at 5,351,669 to 5,352,853 _RS25870 BWI76_RS25875: BWI76_RS25875 - elongation factor G, at 5,352,924 to 5,355,038 _RS25875 Position (kb) 5351 5352 5353Strain fitness (log2 ratio) -2 -1 0 1 2at 5350.682 kb on + strand, within BWI76_RS25850at 5350.685 kb on - strand, within BWI76_RS25850at 5350.888 kb on + strand, within BWI76_RS25855at 5350.909 kb on + strand, within BWI76_RS25855at 5351.057 kb on - strand, within BWI76_RS25855at 5351.075 kb on - strand, within BWI76_RS25855at 5351.126 kb on + strand, within BWI76_RS25855at 5351.240 kb on + strandat 5351.283 kb on + strandat 5351.436 kb on - strandat 5351.473 kb on + strandat 5351.479 kb on - strandat 5351.481 kb on - strandat 5351.483 kb on + strandat 5351.491 kb on - strandat 5351.503 kb on - strand, within BWI76_RS25865at 5351.524 kb on + strand, within BWI76_RS25865at 5351.589 kb on + strandat 5352.505 kb on - strand, within BWI76_RS25870at 5352.856 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction A22 0.1 mg/ml
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5,350,682 + BWI76_RS25850 0.82 +0.5
5,350,685 - BWI76_RS25850 0.83 -1.3
5,350,888 + BWI76_RS25855 0.29 +1.1
5,350,909 + BWI76_RS25855 0.33 +0.7
5,351,057 - BWI76_RS25855 0.64 -0.1
5,351,075 - BWI76_RS25855 0.68 -0.2
5,351,126 + BWI76_RS25855 0.79 -0.0
5,351,240 + +1.9
5,351,283 + +1.1
5,351,436 - -0.2
5,351,473 + +0.4
5,351,479 - +2.5
5,351,481 - +0.3
5,351,483 + +0.3
5,351,491 - -1.8
5,351,503 - BWI76_RS25865 0.50 -0.3
5,351,524 + BWI76_RS25865 0.65 +0.3
5,351,589 + -0.2
5,352,505 - BWI76_RS25870 0.71 -0.8
5,352,856 + -1.4

Or see this region's nucleotide sequence