Experiment: A22 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25850 and BWI76_RS25855 overlap by 17 nucleotides BWI76_RS25855 and BWI76_RS25860 are separated by 74 nucleotides BWI76_RS25860 and BWI76_RS25865 overlap by 60 nucleotides BWI76_RS25865 and BWI76_RS25870 are separated by 98 nucleotides BWI76_RS25870 and BWI76_RS25875 are separated by 70 nucleotides
BWI76_RS25850: BWI76_RS25850 - prepilin peptidase, at 5,350,290 to 5,350,766
_RS25850
BWI76_RS25855: BWI76_RS25855 - bacterioferritin, at 5,350,750 to 5,351,226
_RS25855
BWI76_RS25860: BWI76_RS25860 - bacterioferritin-associated ferredoxin, at 5,351,301 to 5,351,495
_RS25860
BWI76_RS25865: BWI76_RS25865 - ferredoxin, at 5,351,436 to 5,351,570
_RS25865
BWI76_RS25870: BWI76_RS25870 - elongation factor Tu, at 5,351,669 to 5,352,853
_RS25870
BWI76_RS25875: BWI76_RS25875 - elongation factor G, at 5,352,924 to 5,355,038
_RS25875
Position (kb) 5351
5352
5353 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5350.682 kb on + strand, within BWI76_RS25850 at 5350.685 kb on - strand, within BWI76_RS25850 at 5350.888 kb on + strand, within BWI76_RS25855 at 5350.909 kb on + strand, within BWI76_RS25855 at 5351.057 kb on - strand, within BWI76_RS25855 at 5351.075 kb on - strand, within BWI76_RS25855 at 5351.126 kb on + strand, within BWI76_RS25855 at 5351.240 kb on + strand at 5351.283 kb on + strand at 5351.436 kb on - strand at 5351.473 kb on + strand at 5351.479 kb on - strand at 5351.481 kb on - strand at 5351.483 kb on + strand at 5351.491 kb on - strand at 5351.503 kb on - strand, within BWI76_RS25865 at 5351.524 kb on + strand, within BWI76_RS25865 at 5351.589 kb on + strand at 5352.505 kb on - strand, within BWI76_RS25870 at 5352.856 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.1 mg/ml remove 5,350,682 + BWI76_RS25850 0.82 +0.5 5,350,685 - BWI76_RS25850 0.83 -1.3 5,350,888 + BWI76_RS25855 0.29 +1.1 5,350,909 + BWI76_RS25855 0.33 +0.7 5,351,057 - BWI76_RS25855 0.64 -0.1 5,351,075 - BWI76_RS25855 0.68 -0.2 5,351,126 + BWI76_RS25855 0.79 -0.0 5,351,240 + +1.9 5,351,283 + +1.1 5,351,436 - -0.2 5,351,473 + +0.4 5,351,479 - +2.5 5,351,481 - +0.3 5,351,483 + +0.3 5,351,491 - -1.8 5,351,503 - BWI76_RS25865 0.50 -0.3 5,351,524 + BWI76_RS25865 0.65 +0.3 5,351,589 + -0.2 5,352,505 - BWI76_RS25870 0.71 -0.8 5,352,856 + -1.4
Or see this region's nucleotide sequence