Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25225

Experiment: A22 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25215 and BWI76_RS25220 are separated by 18 nucleotidesBWI76_RS25220 and BWI76_RS25225 are separated by 4 nucleotidesBWI76_RS25225 and BWI76_RS25230 are separated by 7 nucleotides BWI76_RS25215: BWI76_RS25215 - phospholipid-binding protein MlaC, at 5,244,400 to 5,245,035 _RS25215 BWI76_RS25220: BWI76_RS25220 - outer membrane lipid asymmetry maintenance protein MlaD, at 5,245,054 to 5,245,605 _RS25220 BWI76_RS25225: BWI76_RS25225 - ABC transporter permease, at 5,245,610 to 5,246,392 _RS25225 BWI76_RS25230: BWI76_RS25230 - phospholipid ABC transporter ATP-binding protein MlaF, at 5,246,400 to 5,247,212 _RS25230 Position (kb) 5245 5246 5247Strain fitness (log2 ratio) -1 0 1at 5244.624 kb on + strand, within BWI76_RS25215at 5244.666 kb on - strand, within BWI76_RS25215at 5244.677 kb on - strand, within BWI76_RS25215at 5244.737 kb on - strand, within BWI76_RS25215at 5244.890 kb on + strand, within BWI76_RS25215at 5244.956 kb on - strand, within BWI76_RS25215at 5245.147 kb on + strand, within BWI76_RS25220at 5245.147 kb on - strand, within BWI76_RS25220at 5245.224 kb on + strand, within BWI76_RS25220at 5245.268 kb on + strand, within BWI76_RS25220at 5245.326 kb on + strand, within BWI76_RS25220at 5245.369 kb on - strand, within BWI76_RS25220at 5245.572 kb on + strandat 5246.024 kb on + strand, within BWI76_RS25225at 5246.161 kb on + strand, within BWI76_RS25225at 5246.241 kb on + strand, within BWI76_RS25225at 5246.241 kb on + strand, within BWI76_RS25225at 5246.252 kb on - strand, within BWI76_RS25225at 5246.294 kb on + strand, within BWI76_RS25225at 5246.338 kb on - strandat 5246.538 kb on - strand, within BWI76_RS25230at 5246.629 kb on + strand, within BWI76_RS25230at 5246.740 kb on + strand, within BWI76_RS25230at 5246.845 kb on - strand, within BWI76_RS25230at 5246.909 kb on + strand, within BWI76_RS25230at 5246.947 kb on - strand, within BWI76_RS25230at 5246.968 kb on + strand, within BWI76_RS25230at 5246.976 kb on - strand, within BWI76_RS25230at 5246.976 kb on - strand, within BWI76_RS25230at 5246.976 kb on - strand, within BWI76_RS25230at 5247.247 kb on - strandat 5247.379 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction A22 0.1 mg/ml
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5,244,624 + BWI76_RS25215 0.35 +0.4
5,244,666 - BWI76_RS25215 0.42 -0.4
5,244,677 - BWI76_RS25215 0.44 -0.6
5,244,737 - BWI76_RS25215 0.53 -0.1
5,244,890 + BWI76_RS25215 0.77 -0.1
5,244,956 - BWI76_RS25215 0.87 +0.0
5,245,147 + BWI76_RS25220 0.17 -0.2
5,245,147 - BWI76_RS25220 0.17 -0.7
5,245,224 + BWI76_RS25220 0.31 +0.4
5,245,268 + BWI76_RS25220 0.39 -0.2
5,245,326 + BWI76_RS25220 0.49 -0.9
5,245,369 - BWI76_RS25220 0.57 -0.9
5,245,572 + -0.6
5,246,024 + BWI76_RS25225 0.53 -0.4
5,246,161 + BWI76_RS25225 0.70 -0.2
5,246,241 + BWI76_RS25225 0.81 -1.6
5,246,241 + BWI76_RS25225 0.81 -0.2
5,246,252 - BWI76_RS25225 0.82 -1.1
5,246,294 + BWI76_RS25225 0.87 -0.4
5,246,338 - -0.3
5,246,538 - BWI76_RS25230 0.17 +0.6
5,246,629 + BWI76_RS25230 0.28 +0.5
5,246,740 + BWI76_RS25230 0.42 +0.1
5,246,845 - BWI76_RS25230 0.55 -0.3
5,246,909 + BWI76_RS25230 0.63 -0.6
5,246,947 - BWI76_RS25230 0.67 -0.8
5,246,968 + BWI76_RS25230 0.70 -0.5
5,246,976 - BWI76_RS25230 0.71 +0.4
5,246,976 - BWI76_RS25230 0.71 -0.2
5,246,976 - BWI76_RS25230 0.71 -0.3
5,247,247 - -0.6
5,247,379 - -0.5

Or see this region's nucleotide sequence