Experiment: A22 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24095 and BWI76_RS24100 are separated by 16 nucleotides BWI76_RS24100 and BWI76_RS24105 are separated by 8 nucleotides BWI76_RS24105 and BWI76_RS24110 overlap by 4 nucleotides BWI76_RS24110 and BWI76_RS24115 are separated by 12 nucleotides
BWI76_RS24095: BWI76_RS24095 - twitching motility protein PilT, at 5,026,489 to 5,027,469
_RS24095
BWI76_RS24100: BWI76_RS24100 - YggS family pyridoxal phosphate enzyme, at 5,027,486 to 5,028,199
_RS24100
BWI76_RS24105: BWI76_RS24105 - resistance protein, at 5,028,208 to 5,028,774
_RS24105
BWI76_RS24110: BWI76_RS24110 - YggU family protein, at 5,028,771 to 5,029,061
_RS24110
BWI76_RS24115: BWI76_RS24115 - non-canonical purine NTP pyrophosphatase, RdgB/HAM1 family, at 5,029,074 to 5,029,667
_RS24115
Position (kb)
5027
5028
5029 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5026.490 kb on + strand at 5026.490 kb on + strand at 5026.495 kb on - strand at 5026.495 kb on - strand at 5026.496 kb on - strand at 5026.498 kb on + strand at 5026.512 kb on - strand at 5026.513 kb on - strand at 5026.515 kb on + strand at 5026.515 kb on + strand at 5026.515 kb on + strand at 5026.515 kb on + strand at 5026.515 kb on + strand at 5026.523 kb on - strand at 5026.523 kb on - strand at 5026.523 kb on - strand at 5026.523 kb on - strand at 5026.523 kb on - strand at 5026.523 kb on - strand at 5026.544 kb on + strand at 5026.544 kb on + strand at 5026.544 kb on + strand at 5026.544 kb on + strand at 5026.544 kb on + strand at 5026.563 kb on + strand at 5026.571 kb on - strand at 5026.586 kb on + strand at 5026.594 kb on - strand, within BWI76_RS24095 at 5026.612 kb on - strand, within BWI76_RS24095 at 5026.622 kb on - strand, within BWI76_RS24095 at 5026.624 kb on - strand, within BWI76_RS24095 at 5026.637 kb on - strand, within BWI76_RS24095 at 5026.647 kb on - strand, within BWI76_RS24095 at 5026.657 kb on - strand, within BWI76_RS24095 at 5026.667 kb on + strand, within BWI76_RS24095 at 5026.776 kb on + strand, within BWI76_RS24095 at 5026.784 kb on - strand, within BWI76_RS24095 at 5026.807 kb on + strand, within BWI76_RS24095 at 5026.819 kb on + strand, within BWI76_RS24095 at 5026.909 kb on + strand, within BWI76_RS24095 at 5026.917 kb on - strand, within BWI76_RS24095 at 5027.021 kb on - strand, within BWI76_RS24095 at 5027.034 kb on + strand, within BWI76_RS24095 at 5027.034 kb on + strand, within BWI76_RS24095 at 5027.034 kb on + strand, within BWI76_RS24095 at 5027.034 kb on + strand, within BWI76_RS24095 at 5027.042 kb on - strand, within BWI76_RS24095 at 5027.100 kb on + strand, within BWI76_RS24095 at 5027.113 kb on + strand, within BWI76_RS24095 at 5027.135 kb on - strand, within BWI76_RS24095 at 5027.309 kb on + strand, within BWI76_RS24095 at 5027.340 kb on + strand, within BWI76_RS24095 at 5027.433 kb on + strand at 5027.446 kb on - strand at 5027.513 kb on - strand at 5027.513 kb on - strand at 5027.568 kb on + strand, within BWI76_RS24100 at 5027.838 kb on - strand, within BWI76_RS24100 at 5027.885 kb on + strand, within BWI76_RS24100 at 5027.888 kb on - strand, within BWI76_RS24100 at 5027.948 kb on - strand, within BWI76_RS24100 at 5027.963 kb on - strand, within BWI76_RS24100 at 5028.060 kb on - strand, within BWI76_RS24100 at 5028.132 kb on + strand at 5028.140 kb on - strand at 5028.140 kb on - strand at 5028.413 kb on + strand, within BWI76_RS24105 at 5028.413 kb on + strand, within BWI76_RS24105 at 5028.421 kb on - strand, within BWI76_RS24105 at 5028.421 kb on - strand, within BWI76_RS24105 at 5028.447 kb on + strand, within BWI76_RS24105 at 5028.447 kb on + strand, within BWI76_RS24105 at 5028.560 kb on + strand, within BWI76_RS24105 at 5028.723 kb on + strand at 5028.759 kb on + strand at 5028.824 kb on + strand, within BWI76_RS24110 at 5028.824 kb on + strand, within BWI76_RS24110 at 5029.038 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.1 mg/ml remove 5,026,490 + -0.7 5,026,490 + +1.5 5,026,495 - -0.8 5,026,495 - +1.7 5,026,496 - +0.7 5,026,498 + +0.3 5,026,512 - +0.9 5,026,513 - -0.5 5,026,515 + +0.1 5,026,515 + -1.2 5,026,515 + +1.7 5,026,515 + -0.8 5,026,515 + +0.2 5,026,523 - +1.4 5,026,523 - -0.1 5,026,523 - +2.9 5,026,523 - -0.8 5,026,523 - +0.2 5,026,523 - +0.1 5,026,544 + -0.5 5,026,544 + -0.2 5,026,544 + +0.8 5,026,544 + +0.0 5,026,544 + -0.1 5,026,563 + +0.2 5,026,571 - +0.3 5,026,586 + -1.2 5,026,594 - BWI76_RS24095 0.11 +0.5 5,026,612 - BWI76_RS24095 0.13 +0.1 5,026,622 - BWI76_RS24095 0.14 -0.3 5,026,624 - BWI76_RS24095 0.14 -0.4 5,026,637 - BWI76_RS24095 0.15 -1.7 5,026,647 - BWI76_RS24095 0.16 -1.3 5,026,657 - BWI76_RS24095 0.17 -0.1 5,026,667 + BWI76_RS24095 0.18 -1.4 5,026,776 + BWI76_RS24095 0.29 -0.1 5,026,784 - BWI76_RS24095 0.30 -0.0 5,026,807 + BWI76_RS24095 0.32 -0.1 5,026,819 + BWI76_RS24095 0.34 -0.5 5,026,909 + BWI76_RS24095 0.43 -1.8 5,026,917 - BWI76_RS24095 0.44 +0.2 5,027,021 - BWI76_RS24095 0.54 -1.0 5,027,034 + BWI76_RS24095 0.56 +0.7 5,027,034 + BWI76_RS24095 0.56 -0.1 5,027,034 + BWI76_RS24095 0.56 -1.1 5,027,034 + BWI76_RS24095 0.56 +0.3 5,027,042 - BWI76_RS24095 0.56 +0.2 5,027,100 + BWI76_RS24095 0.62 +0.8 5,027,113 + BWI76_RS24095 0.64 -0.5 5,027,135 - BWI76_RS24095 0.66 +0.2 5,027,309 + BWI76_RS24095 0.84 +0.1 5,027,340 + BWI76_RS24095 0.87 -0.5 5,027,433 + +0.3 5,027,446 - -0.8 5,027,513 - -0.8 5,027,513 - -1.2 5,027,568 + BWI76_RS24100 0.11 -0.6 5,027,838 - BWI76_RS24100 0.49 -0.1 5,027,885 + BWI76_RS24100 0.56 -0.2 5,027,888 - BWI76_RS24100 0.56 -0.5 5,027,948 - BWI76_RS24100 0.65 -2.4 5,027,963 - BWI76_RS24100 0.67 -1.0 5,028,060 - BWI76_RS24100 0.80 -0.9 5,028,132 + -0.6 5,028,140 - -2.1 5,028,140 - -0.2 5,028,413 + BWI76_RS24105 0.36 +1.7 5,028,413 + BWI76_RS24105 0.36 +0.7 5,028,421 - BWI76_RS24105 0.38 +0.2 5,028,421 - BWI76_RS24105 0.38 +0.7 5,028,447 + BWI76_RS24105 0.42 -0.0 5,028,447 + BWI76_RS24105 0.42 -0.5 5,028,560 + BWI76_RS24105 0.62 +1.9 5,028,723 + +0.2 5,028,759 + -0.1 5,028,824 + BWI76_RS24110 0.18 -0.5 5,028,824 + BWI76_RS24110 0.18 -0.1 5,029,038 - -0.9
Or see this region's nucleotide sequence