Experiment: A22 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23235 and BWI76_RS23240 are separated by 128 nucleotides BWI76_RS23240 and BWI76_RS23245 are separated by 12 nucleotides BWI76_RS23245 and BWI76_RS23250 overlap by 16 nucleotides BWI76_RS23250 and BWI76_RS23255 overlap by 4 nucleotides
BWI76_RS23235: BWI76_RS23235 - pitrilysin, at 4,843,827 to 4,846,712
_RS23235
BWI76_RS23240: BWI76_RS23240 - exonuclease V subunit gamma, at 4,846,841 to 4,850,215
_RS23240
BWI76_RS23245: BWI76_RS23245 - hypothetical protein, at 4,850,228 to 4,850,551
_RS23245
BWI76_RS23250: BWI76_RS23250 - hypothetical protein, at 4,850,536 to 4,850,943
_RS23250
BWI76_RS23255: BWI76_RS23255 - prepilin-type cleavage/methylation domain-containing protein, at 4,850,940 to 4,851,503
_RS23255
Position (kb) 4846
4847
4848
4849
4850
4851 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4846.713 kb on + strand at 4847.810 kb on + strand, within BWI76_RS23240 at 4848.002 kb on - strand, within BWI76_RS23240 at 4848.397 kb on + strand, within BWI76_RS23240 at 4848.798 kb on + strand, within BWI76_RS23240 at 4848.932 kb on + strand, within BWI76_RS23240 at 4848.940 kb on - strand, within BWI76_RS23240 at 4849.704 kb on + strand, within BWI76_RS23240 at 4849.712 kb on - strand, within BWI76_RS23240 at 4850.375 kb on - strand, within BWI76_RS23245 at 4850.433 kb on + strand, within BWI76_RS23245 at 4850.679 kb on - strand, within BWI76_RS23250 at 4850.784 kb on - strand, within BWI76_RS23250 at 4850.953 kb on - strand at 4851.173 kb on + strand, within BWI76_RS23255
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.1 mg/ml remove 4,846,713 + +0.2 4,847,810 + BWI76_RS23240 0.29 -2.4 4,848,002 - BWI76_RS23240 0.34 -2.6 4,848,397 + BWI76_RS23240 0.46 -1.4 4,848,798 + BWI76_RS23240 0.58 -3.4 4,848,932 + BWI76_RS23240 0.62 -2.1 4,848,940 - BWI76_RS23240 0.62 -2.6 4,849,704 + BWI76_RS23240 0.85 -2.7 4,849,712 - BWI76_RS23240 0.85 -1.9 4,850,375 - BWI76_RS23245 0.45 +0.5 4,850,433 + BWI76_RS23245 0.63 -0.3 4,850,679 - BWI76_RS23250 0.35 +0.3 4,850,784 - BWI76_RS23250 0.61 -0.4 4,850,953 - -0.0 4,851,173 + BWI76_RS23255 0.41 +1.0
Or see this region's nucleotide sequence