Experiment: A22 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21370 and BWI76_RS21375 are separated by 44 nucleotides BWI76_RS21375 and BWI76_RS21380 are separated by 311 nucleotides BWI76_RS21380 and BWI76_RS21385 are separated by 55 nucleotides
BWI76_RS21370: BWI76_RS21370 - ATP-dependent RNA helicase SrmB, at 4,473,569 to 4,474,900
_RS21370
BWI76_RS21375: BWI76_RS21375 - autonomous glycyl radical cofactor GrcA, at 4,474,945 to 4,475,328
_RS21375
BWI76_RS21380: BWI76_RS21380 - uracil-DNA glycosylase, at 4,475,640 to 4,476,329
_RS21380
BWI76_RS21385: BWI76_RS21385 - tRNA/rRNA methyltransferase, at 4,476,385 to 4,477,458
_RS21385
Position (kb) 4475
4476
4477 Strain fitness (log2 ratio) -1
0
1 at 4475.120 kb on - strand, within BWI76_RS21375 at 4475.276 kb on - strand, within BWI76_RS21375 at 4475.409 kb on - strand at 4475.499 kb on + strand at 4475.796 kb on - strand, within BWI76_RS21380 at 4475.819 kb on - strand, within BWI76_RS21380 at 4476.449 kb on - strand at 4476.457 kb on - strand at 4476.489 kb on - strand at 4476.489 kb on - strand at 4476.566 kb on + strand, within BWI76_RS21385 at 4476.574 kb on - strand, within BWI76_RS21385 at 4476.862 kb on - strand, within BWI76_RS21385 at 4477.005 kb on + strand, within BWI76_RS21385 at 4477.019 kb on - strand, within BWI76_RS21385 at 4477.205 kb on - strand, within BWI76_RS21385 at 4477.231 kb on - strand, within BWI76_RS21385
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.1 mg/ml remove 4,475,120 - BWI76_RS21375 0.46 -1.6 4,475,276 - BWI76_RS21375 0.86 -0.6 4,475,409 - -0.7 4,475,499 + +0.3 4,475,796 - BWI76_RS21380 0.23 +0.5 4,475,819 - BWI76_RS21380 0.26 +0.2 4,476,449 - -0.4 4,476,457 - -0.2 4,476,489 - -0.0 4,476,489 - -0.8 4,476,566 + BWI76_RS21385 0.17 -1.5 4,476,574 - BWI76_RS21385 0.18 -0.5 4,476,862 - BWI76_RS21385 0.44 -0.2 4,477,005 + BWI76_RS21385 0.58 -0.5 4,477,019 - BWI76_RS21385 0.59 -0.1 4,477,205 - BWI76_RS21385 0.76 +0.1 4,477,231 - BWI76_RS21385 0.79 +0.1
Or see this region's nucleotide sequence