Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16150

Experiment: A22 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16140 and BWI76_RS16145 are separated by 10 nucleotidesBWI76_RS16145 and BWI76_RS16150 are separated by 167 nucleotidesBWI76_RS16150 and BWI76_RS16155 overlap by 4 nucleotidesBWI76_RS16155 and BWI76_RS16160 are separated by 101 nucleotides BWI76_RS16140: BWI76_RS16140 - 3-oxoadipate CoA-transferase, at 3,348,963 to 3,349,619 _RS16140 BWI76_RS16145: BWI76_RS16145 - 3-oxoadipate CoA-transferase, at 3,349,630 to 3,350,316 _RS16145 BWI76_RS16150: BWI76_RS16150 - IclR family transcriptional regulator, at 3,350,484 to 3,351,290 _RS16150 BWI76_RS16155: BWI76_RS16155 - hypothetical protein, at 3,351,287 to 3,351,850 _RS16155 BWI76_RS16160: BWI76_RS16160 - LysR family transcriptional regulator, at 3,351,952 to 3,352,860 _RS16160 Position (kb) 3350 3351 3352Strain fitness (log2 ratio) -1 0 1at 3350.569 kb on + strand, within BWI76_RS16150at 3350.577 kb on - strand, within BWI76_RS16150at 3351.301 kb on - strandat 3351.925 kb on + strandat 3351.929 kb on - strandat 3351.978 kb on - strandat 3352.211 kb on + strand, within BWI76_RS16160

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Per-strain Table

Position Strand Gene LocusTag Fraction A22 0.1 mg/ml
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3,350,569 + BWI76_RS16150 0.11 -1.0
3,350,577 - BWI76_RS16150 0.12 +0.4
3,351,301 - +0.7
3,351,925 + -1.2
3,351,929 - +0.1
3,351,978 - +0.4
3,352,211 + BWI76_RS16160 0.28 -0.4

Or see this region's nucleotide sequence