Experiment: A22 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16140 and BWI76_RS16145 are separated by 10 nucleotides BWI76_RS16145 and BWI76_RS16150 are separated by 167 nucleotides BWI76_RS16150 and BWI76_RS16155 overlap by 4 nucleotides BWI76_RS16155 and BWI76_RS16160 are separated by 101 nucleotides
BWI76_RS16140: BWI76_RS16140 - 3-oxoadipate CoA-transferase, at 3,348,963 to 3,349,619
_RS16140
BWI76_RS16145: BWI76_RS16145 - 3-oxoadipate CoA-transferase, at 3,349,630 to 3,350,316
_RS16145
BWI76_RS16150: BWI76_RS16150 - IclR family transcriptional regulator, at 3,350,484 to 3,351,290
_RS16150
BWI76_RS16155: BWI76_RS16155 - hypothetical protein, at 3,351,287 to 3,351,850
_RS16155
BWI76_RS16160: BWI76_RS16160 - LysR family transcriptional regulator, at 3,351,952 to 3,352,860
_RS16160
Position (kb)
3350
3351
3352 Strain fitness (log2 ratio)
-1
0
1 at 3350.569 kb on + strand, within BWI76_RS16150 at 3350.577 kb on - strand, within BWI76_RS16150 at 3351.301 kb on - strand at 3351.925 kb on + strand at 3351.929 kb on - strand at 3351.978 kb on - strand at 3352.211 kb on + strand, within BWI76_RS16160
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.1 mg/ml remove 3,350,569 + BWI76_RS16150 0.11 -1.0 3,350,577 - BWI76_RS16150 0.12 +0.4 3,351,301 - +0.7 3,351,925 + -1.2 3,351,929 - +0.1 3,351,978 - +0.4 3,352,211 + BWI76_RS16160 0.28 -0.4
Or see this region's nucleotide sequence