Experiment: A22 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14400 and BWI76_RS14405 are separated by 120 nucleotides BWI76_RS14405 and BWI76_RS14410 are separated by 91 nucleotides BWI76_RS14410 and BWI76_RS14415 are separated by 118 nucleotides BWI76_RS14415 and BWI76_RS14420 are separated by 17 nucleotides
BWI76_RS14400: BWI76_RS14400 - ISNCY family transposase, at 3,008,628 to 3,009,198
_RS14400
BWI76_RS14405: BWI76_RS14405 - steroid Delta-isomerase, at 3,009,319 to 3,009,672
_RS14405
BWI76_RS14410: BWI76_RS14410 - hypothetical protein, at 3,009,764 to 3,009,967
_RS14410
BWI76_RS14415: BWI76_RS14415 - transcriptional regulator, TetR family protein, at 3,010,086 to 3,010,682
_RS14415
BWI76_RS14420: BWI76_RS14420 - hypothetical protein, at 3,010,700 to 3,011,482
_RS14420
Position (kb)
3009
3010 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3008.909 kb on + strand, within BWI76_RS14400 at 3008.909 kb on + strand, within BWI76_RS14400 at 3008.959 kb on - strand, within BWI76_RS14400 at 3009.019 kb on - strand, within BWI76_RS14400 at 3009.084 kb on + strand, within BWI76_RS14400 at 3009.096 kb on + strand, within BWI76_RS14400 at 3009.096 kb on + strand, within BWI76_RS14400 at 3009.096 kb on + strand, within BWI76_RS14400 at 3009.096 kb on + strand, within BWI76_RS14400 at 3009.096 kb on + strand, within BWI76_RS14400 at 3009.130 kb on - strand, within BWI76_RS14400 at 3009.130 kb on - strand, within BWI76_RS14400 at 3009.178 kb on + strand at 3009.178 kb on + strand at 3009.178 kb on + strand at 3009.251 kb on + strand at 3009.264 kb on - strand at 3009.766 kb on - strand at 3009.812 kb on - strand, within BWI76_RS14410 at 3009.822 kb on - strand, within BWI76_RS14410 at 3009.822 kb on - strand, within BWI76_RS14410 at 3009.822 kb on - strand, within BWI76_RS14410 at 3009.848 kb on + strand, within BWI76_RS14410 at 3009.856 kb on - strand at 3009.962 kb on + strand at 3009.970 kb on - strand at 3009.970 kb on - strand at 3009.986 kb on + strand at 3009.989 kb on - strand at 3009.994 kb on - strand at 3009.994 kb on - strand at 3009.994 kb on - strand at 3009.994 kb on - strand at 3010.128 kb on + strand at 3010.341 kb on - strand, within BWI76_RS14415 at 3010.400 kb on + strand, within BWI76_RS14415 at 3010.401 kb on - strand, within BWI76_RS14415 at 3010.401 kb on - strand, within BWI76_RS14415 at 3010.544 kb on - strand, within BWI76_RS14415 at 3010.667 kb on - strand at 3010.757 kb on + strand at 3010.760 kb on - strand at 3010.782 kb on + strand, within BWI76_RS14420 at 3010.782 kb on + strand, within BWI76_RS14420 at 3010.782 kb on + strand, within BWI76_RS14420 at 3010.782 kb on + strand, within BWI76_RS14420 at 3010.782 kb on + strand, within BWI76_RS14420 at 3010.782 kb on + strand, within BWI76_RS14420 at 3010.782 kb on + strand, within BWI76_RS14420 at 3010.790 kb on - strand, within BWI76_RS14420 at 3010.790 kb on - strand, within BWI76_RS14420 at 3010.797 kb on + strand, within BWI76_RS14420 at 3010.797 kb on + strand, within BWI76_RS14420 at 3010.797 kb on + strand, within BWI76_RS14420 at 3010.797 kb on + strand, within BWI76_RS14420 at 3010.803 kb on + strand, within BWI76_RS14420 at 3010.805 kb on - strand, within BWI76_RS14420 at 3010.805 kb on - strand, within BWI76_RS14420 at 3010.908 kb on - strand, within BWI76_RS14420 at 3010.929 kb on - strand, within BWI76_RS14420 at 3010.929 kb on - strand, within BWI76_RS14420 at 3010.934 kb on - strand, within BWI76_RS14420 at 3010.934 kb on - strand, within BWI76_RS14420 at 3010.934 kb on - strand, within BWI76_RS14420 at 3010.934 kb on - strand, within BWI76_RS14420 at 3010.934 kb on - strand, within BWI76_RS14420 at 3010.934 kb on - strand, within BWI76_RS14420 at 3010.934 kb on - strand, within BWI76_RS14420
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.1 mg/ml remove 3,008,909 + BWI76_RS14400 0.49 +0.2 3,008,909 + BWI76_RS14400 0.49 +0.4 3,008,959 - BWI76_RS14400 0.58 -1.6 3,009,019 - BWI76_RS14400 0.68 -0.0 3,009,084 + BWI76_RS14400 0.80 -0.0 3,009,096 + BWI76_RS14400 0.82 -0.9 3,009,096 + BWI76_RS14400 0.82 +1.1 3,009,096 + BWI76_RS14400 0.82 +0.2 3,009,096 + BWI76_RS14400 0.82 +0.4 3,009,096 + BWI76_RS14400 0.82 +1.6 3,009,130 - BWI76_RS14400 0.88 +0.1 3,009,130 - BWI76_RS14400 0.88 -0.9 3,009,178 + -0.8 3,009,178 + +0.5 3,009,178 + +0.8 3,009,251 + +0.8 3,009,264 - -0.8 3,009,766 - -0.7 3,009,812 - BWI76_RS14410 0.24 -0.0 3,009,822 - BWI76_RS14410 0.28 +1.4 3,009,822 - BWI76_RS14410 0.28 +0.1 3,009,822 - BWI76_RS14410 0.28 -1.4 3,009,848 + BWI76_RS14410 0.41 -1.3 3,009,856 - -0.4 3,009,962 + -0.7 3,009,970 - -1.0 3,009,970 - +0.4 3,009,986 + -1.4 3,009,989 - -0.3 3,009,994 - +1.3 3,009,994 - -0.0 3,009,994 - +0.2 3,009,994 - -1.4 3,010,128 + -1.2 3,010,341 - BWI76_RS14415 0.43 +0.6 3,010,400 + BWI76_RS14415 0.53 +2.6 3,010,401 - BWI76_RS14415 0.53 -0.0 3,010,401 - BWI76_RS14415 0.53 +0.1 3,010,544 - BWI76_RS14415 0.77 +0.6 3,010,667 - +2.3 3,010,757 + -0.8 3,010,760 - +0.7 3,010,782 + BWI76_RS14420 0.10 +0.6 3,010,782 + BWI76_RS14420 0.10 +1.6 3,010,782 + BWI76_RS14420 0.10 +0.2 3,010,782 + BWI76_RS14420 0.10 -0.2 3,010,782 + BWI76_RS14420 0.10 +1.2 3,010,782 + BWI76_RS14420 0.10 +2.3 3,010,782 + BWI76_RS14420 0.10 +1.0 3,010,790 - BWI76_RS14420 0.11 +1.6 3,010,790 - BWI76_RS14420 0.11 +0.4 3,010,797 + BWI76_RS14420 0.12 +1.6 3,010,797 + BWI76_RS14420 0.12 +1.1 3,010,797 + BWI76_RS14420 0.12 -1.9 3,010,797 + BWI76_RS14420 0.12 -1.7 3,010,803 + BWI76_RS14420 0.13 +0.6 3,010,805 - BWI76_RS14420 0.13 -1.9 3,010,805 - BWI76_RS14420 0.13 -0.6 3,010,908 - BWI76_RS14420 0.27 +0.4 3,010,929 - BWI76_RS14420 0.29 -1.2 3,010,929 - BWI76_RS14420 0.29 -0.6 3,010,934 - BWI76_RS14420 0.30 +0.4 3,010,934 - BWI76_RS14420 0.30 +0.1 3,010,934 - BWI76_RS14420 0.30 +0.7 3,010,934 - BWI76_RS14420 0.30 -0.2 3,010,934 - BWI76_RS14420 0.30 +0.8 3,010,934 - BWI76_RS14420 0.30 -0.1 3,010,934 - BWI76_RS14420 0.30 -0.0
Or see this region's nucleotide sequence