Experiment: A22 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12415 and BWI76_RS12420 overlap by 4 nucleotides BWI76_RS12420 and BWI76_RS12425 are separated by 363 nucleotides BWI76_RS12425 and BWI76_RS12430 are separated by 169 nucleotides
BWI76_RS12415: BWI76_RS12415 - antimicrobial peptide ABC transporter permease SapB, at 2,593,043 to 2,594,008
_RS12415
BWI76_RS12420: BWI76_RS12420 - peptide ABC transporter substrate-binding protein SapA, at 2,594,005 to 2,595,648
_RS12420
BWI76_RS12425: BWI76_RS12425 - hypothetical protein, at 2,596,012 to 2,596,296
_RS12425
BWI76_RS12430: BWI76_RS12430 - AraC family transcriptional regulator, at 2,596,466 to 2,598,772
_RS12430
Position (kb)
2594
2595
2596 Strain fitness (log2 ratio)
-2
-1
0
1 at 2594.126 kb on - strand at 2594.815 kb on - strand, within BWI76_RS12420 at 2595.881 kb on + strand at 2595.889 kb on - strand at 2595.889 kb on - strand at 2595.889 kb on - strand at 2596.004 kb on - strand at 2596.086 kb on - strand, within BWI76_RS12425 at 2596.105 kb on - strand, within BWI76_RS12425 at 2596.115 kb on - strand, within BWI76_RS12425 at 2596.244 kb on + strand, within BWI76_RS12425 at 2596.252 kb on - strand, within BWI76_RS12425 at 2596.594 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.1 mg/ml remove 2,594,126 - -0.1 2,594,815 - BWI76_RS12420 0.49 -1.9 2,595,881 + +0.4 2,595,889 - +0.2 2,595,889 - +0.1 2,595,889 - +0.2 2,596,004 - -0.2 2,596,086 - BWI76_RS12425 0.26 +0.4 2,596,105 - BWI76_RS12425 0.33 -0.2 2,596,115 - BWI76_RS12425 0.36 -0.2 2,596,244 + BWI76_RS12425 0.81 -0.4 2,596,252 - BWI76_RS12425 0.84 +0.2 2,596,594 + -0.1
Or see this region's nucleotide sequence