Experiment: A22 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08385 and BWI76_RS08390 are separated by 437 nucleotides BWI76_RS08390 and BWI76_RS08395 overlap by 17 nucleotides BWI76_RS08395 and BWI76_RS08400 overlap by 7 nucleotides BWI76_RS08400 and BWI76_RS08405 overlap by 1 nucleotides
BWI76_RS08385: BWI76_RS08385 - citrate (Si)-synthase, at 1,773,678 to 1,774,961
_RS08385
BWI76_RS08390: BWI76_RS08390 - hypothetical protein, at 1,775,399 to 1,775,617
_RS08390
BWI76_RS08395: BWI76_RS08395 - succinate dehydrogenase cytochrome b556 large subunit, at 1,775,601 to 1,776,005
_RS08395
BWI76_RS08400: BWI76_RS08400 - succinate dehydrogenase cytochrome b556 small membrane subunit, at 1,775,999 to 1,776,346
_RS08400
BWI76_RS08405: BWI76_RS08405 - succinate dehydrogenase flavoprotein subunit, at 1,776,346 to 1,778,112
_RS08405
Position (kb)
1775
1776
1777 Strain fitness (log2 ratio)
-1
0
1 at 1774.604 kb on - strand, within BWI76_RS08385 at 1775.091 kb on + strand at 1775.099 kb on - strand at 1775.114 kb on + strand at 1775.183 kb on + strand at 1775.196 kb on - strand at 1775.207 kb on + strand at 1775.215 kb on + strand at 1775.450 kb on + strand, within BWI76_RS08390 at 1775.461 kb on - strand, within BWI76_RS08390 at 1775.658 kb on - strand at 1775.929 kb on - strand, within BWI76_RS08395 at 1776.180 kb on - strand, within BWI76_RS08400 at 1776.331 kb on + strand at 1776.374 kb on - strand at 1776.384 kb on - strand at 1776.438 kb on + strand at 1776.569 kb on + strand, within BWI76_RS08405 at 1776.836 kb on + strand, within BWI76_RS08405 at 1776.973 kb on - strand, within BWI76_RS08405
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.1 mg/ml remove 1,774,604 - BWI76_RS08385 0.72 -1.0 1,775,091 + -1.0 1,775,099 - +0.0 1,775,114 + -0.6 1,775,183 + +0.4 1,775,196 - -0.1 1,775,207 + -1.2 1,775,215 + +0.2 1,775,450 + BWI76_RS08390 0.23 -0.0 1,775,461 - BWI76_RS08390 0.28 -0.5 1,775,658 - -0.2 1,775,929 - BWI76_RS08395 0.81 -1.3 1,776,180 - BWI76_RS08400 0.52 +0.1 1,776,331 + +0.2 1,776,374 - +0.3 1,776,384 - +0.4 1,776,438 + -1.6 1,776,569 + BWI76_RS08405 0.13 -0.8 1,776,836 + BWI76_RS08405 0.28 +0.4 1,776,973 - BWI76_RS08405 0.35 +0.2
Or see this region's nucleotide sequence