Experiment: A22 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08235 and BWI76_RS08240 are separated by 46 nucleotides BWI76_RS08240 and BWI76_RS08245 are separated by 52 nucleotides BWI76_RS08245 and BWI76_RS08250 are separated by 52 nucleotides
BWI76_RS08235: BWI76_RS08235 - chitoporin, at 1,743,269 to 1,744,672
_RS08235
BWI76_RS08240: BWI76_RS08240 - hypothetical protein, at 1,744,719 to 1,745,051
_RS08240
BWI76_RS08245: BWI76_RS08245 - MFS transporter, at 1,745,104 to 1,746,399
_RS08245
BWI76_RS08250: BWI76_RS08250 - tricarballylate utilization protein B, at 1,746,452 to 1,747,591
_RS08250
Position (kb)
1745
1746
1747 Strain fitness (log2 ratio)
-1
0
1
2 at 1745.045 kb on + strand at 1745.390 kb on - strand, within BWI76_RS08245 at 1745.401 kb on - strand, within BWI76_RS08245 at 1745.440 kb on + strand, within BWI76_RS08245 at 1745.456 kb on + strand, within BWI76_RS08245 at 1745.570 kb on + strand, within BWI76_RS08245 at 1745.754 kb on + strand at 1746.007 kb on + strand at 1746.365 kb on - strand at 1746.439 kb on + strand at 1746.452 kb on - strand at 1746.739 kb on + strand, within BWI76_RS08250 at 1746.936 kb on - strand, within BWI76_RS08250 at 1746.936 kb on - strand, within BWI76_RS08250 at 1747.282 kb on - strand, within BWI76_RS08250
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.1 mg/ml remove 1,745,045 + -0.2 1,745,390 - BWI76_RS08245 0.22 +0.8 1,745,401 - BWI76_RS08245 0.23 +1.8 1,745,440 + BWI76_RS08245 0.26 +0.4 1,745,456 + BWI76_RS08245 0.27 -0.5 1,745,570 + BWI76_RS08245 0.36 -0.1 1,745,754 + +1.5 1,746,007 + -0.5 1,746,365 - -0.5 1,746,439 + -0.2 1,746,452 - +0.8 1,746,739 + BWI76_RS08250 0.25 -0.1 1,746,936 - BWI76_RS08250 0.42 +0.2 1,746,936 - BWI76_RS08250 0.42 +0.1 1,747,282 - BWI76_RS08250 0.73 +0.5
Or see this region's nucleotide sequence