Experiment: A22 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07065 and BWI76_RS07070 overlap by 4 nucleotides BWI76_RS07070 and BWI76_RS07075 are separated by 96 nucleotides BWI76_RS07075 and BWI76_RS07080 are separated by 106 nucleotides BWI76_RS07080 and BWI76_RS07085 are separated by 3 nucleotides
BWI76_RS07065: BWI76_RS07065 - 5-(carboxyamino)imidazole ribonucleotide synthase, at 1,486,550 to 1,487,617
_RS07065
BWI76_RS07070: BWI76_RS07070 - 5-(carboxyamino)imidazole ribonucleotide mutase, at 1,487,614 to 1,488,123
_RS07070
BWI76_RS07075: BWI76_RS07075 - hypothetical protein, at 1,488,220 to 1,488,549
_RS07075
BWI76_RS07080: BWI76_RS07080 - UDP-2,3-diacylglucosamine diphosphatase, at 1,488,656 to 1,489,378
_RS07080
BWI76_RS07085: BWI76_RS07085 - peptidyl-prolyl cis-trans isomerase, at 1,489,382 to 1,489,876
_RS07085
Position (kb)
1488
1489 Strain fitness (log2 ratio)
-1
0
1
2 at 1487.222 kb on + strand, within BWI76_RS07065 at 1487.293 kb on - strand, within BWI76_RS07065 at 1487.293 kb on - strand, within BWI76_RS07065 at 1487.639 kb on - strand at 1487.641 kb on + strand at 1487.694 kb on + strand, within BWI76_RS07070 at 1487.835 kb on - strand, within BWI76_RS07070 at 1487.847 kb on - strand, within BWI76_RS07070 at 1487.901 kb on - strand, within BWI76_RS07070 at 1487.962 kb on - strand, within BWI76_RS07070 at 1488.071 kb on + strand, within BWI76_RS07070 at 1488.148 kb on + strand at 1488.156 kb on - strand at 1488.158 kb on + strand at 1488.158 kb on + strand at 1488.344 kb on + strand, within BWI76_RS07075 at 1488.344 kb on + strand, within BWI76_RS07075 at 1488.375 kb on + strand, within BWI76_RS07075 at 1488.435 kb on + strand, within BWI76_RS07075 at 1489.529 kb on - strand, within BWI76_RS07085
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.1 mg/ml remove 1,487,222 + BWI76_RS07065 0.63 -0.4 1,487,293 - BWI76_RS07065 0.70 -0.0 1,487,293 - BWI76_RS07065 0.70 +0.1 1,487,639 - -0.2 1,487,641 + -0.6 1,487,694 + BWI76_RS07070 0.16 +0.1 1,487,835 - BWI76_RS07070 0.43 -1.1 1,487,847 - BWI76_RS07070 0.46 -0.5 1,487,901 - BWI76_RS07070 0.56 +0.6 1,487,962 - BWI76_RS07070 0.68 +1.9 1,488,071 + BWI76_RS07070 0.90 +1.5 1,488,148 + -0.7 1,488,156 - -0.5 1,488,158 + -1.4 1,488,158 + +1.3 1,488,344 + BWI76_RS07075 0.38 -0.5 1,488,344 + BWI76_RS07075 0.38 -0.6 1,488,375 + BWI76_RS07075 0.47 -1.2 1,488,435 + BWI76_RS07075 0.65 -0.6 1,489,529 - BWI76_RS07085 0.30 -0.2
Or see this region's nucleotide sequence