Experiment: A22 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05940 and BWI76_RS05945 are separated by 27 nucleotides BWI76_RS05945 and BWI76_RS05950 overlap by 11 nucleotides BWI76_RS05950 and BWI76_RS05955 overlap by 4 nucleotides BWI76_RS05955 and BWI76_RS05960 are separated by 13 nucleotides
BWI76_RS05940: BWI76_RS05940 - hypothetical protein, at 1,249,636 to 1,250,340
_RS05940
BWI76_RS05945: BWI76_RS05945 - amino acid ABC transporter permease, at 1,250,368 to 1,251,051
_RS05945
BWI76_RS05950: BWI76_RS05950 - polar amino acid ABC transporter inner membrane subunit, at 1,251,041 to 1,251,793
_RS05950
BWI76_RS05955: BWI76_RS05955 - ABC transporter, at 1,251,790 to 1,252,608
_RS05955
BWI76_RS05960: BWI76_RS05960 - cysteine transport system periplasmic binding protein, at 1,252,622 to 1,253,431
_RS05960
Position (kb)
1251
1252 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1250.122 kb on + strand, within BWI76_RS05940 at 1250.176 kb on - strand, within BWI76_RS05940 at 1250.291 kb on - strand at 1250.293 kb on - strand at 1250.317 kb on + strand at 1250.427 kb on - strand at 1250.465 kb on + strand, within BWI76_RS05945 at 1250.465 kb on + strand, within BWI76_RS05945 at 1250.473 kb on - strand, within BWI76_RS05945 at 1250.554 kb on - strand, within BWI76_RS05945 at 1250.596 kb on - strand, within BWI76_RS05945 at 1250.729 kb on + strand, within BWI76_RS05945 at 1250.929 kb on - strand, within BWI76_RS05945 at 1251.378 kb on - strand, within BWI76_RS05950 at 1252.054 kb on - strand, within BWI76_RS05955 at 1252.243 kb on + strand, within BWI76_RS05955 at 1252.419 kb on - strand, within BWI76_RS05955 at 1252.780 kb on + strand, within BWI76_RS05960
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.1 mg/ml remove 1,250,122 + BWI76_RS05940 0.69 +0.3 1,250,176 - BWI76_RS05940 0.77 +0.3 1,250,291 - +0.1 1,250,293 - -0.5 1,250,317 + +0.3 1,250,427 - -0.4 1,250,465 + BWI76_RS05945 0.14 +1.7 1,250,465 + BWI76_RS05945 0.14 -1.1 1,250,473 - BWI76_RS05945 0.15 +0.1 1,250,554 - BWI76_RS05945 0.27 +0.8 1,250,596 - BWI76_RS05945 0.33 -0.9 1,250,729 + BWI76_RS05945 0.53 +0.1 1,250,929 - BWI76_RS05945 0.82 +2.9 1,251,378 - BWI76_RS05950 0.45 -1.2 1,252,054 - BWI76_RS05955 0.32 +1.1 1,252,243 + BWI76_RS05955 0.55 -0.6 1,252,419 - BWI76_RS05955 0.77 +0.1 1,252,780 + BWI76_RS05960 0.20 -0.7
Or see this region's nucleotide sequence