Experiment: A22 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05470 and BWI76_RS05475 are separated by 238 nucleotides BWI76_RS05475 and BWI76_RS05480 are separated by 43 nucleotides BWI76_RS05480 and BWI76_RS05485 overlap by 30 nucleotides
BWI76_RS05470: BWI76_RS05470 - acyl-CoA dehydrogenase, at 1,146,634 to 1,149,078
_RS05470
BWI76_RS05475: BWI76_RS05475 - phosphoheptose isomerase, at 1,149,317 to 1,149,898
_RS05475
BWI76_RS05480: BWI76_RS05480 - class II glutamine amidotransferase, at 1,149,942 to 1,150,709
_RS05480
BWI76_RS05485: BWI76_RS05485 - transpeptidase, at 1,150,680 to 1,151,420
_RS05485
Position (kb)
1149
1150
1151 Strain fitness (log2 ratio)
-1
0
1
2 at 1149.270 kb on + strand at 1150.067 kb on + strand, within BWI76_RS05480 at 1150.068 kb on + strand, within BWI76_RS05480 at 1150.076 kb on - strand, within BWI76_RS05480 at 1150.252 kb on - strand, within BWI76_RS05480 at 1150.474 kb on - strand, within BWI76_RS05480 at 1151.107 kb on + strand, within BWI76_RS05485 at 1151.179 kb on - strand, within BWI76_RS05485 at 1151.179 kb on - strand, within BWI76_RS05485 at 1151.186 kb on + strand, within BWI76_RS05485 at 1151.214 kb on + strand, within BWI76_RS05485 at 1151.284 kb on + strand, within BWI76_RS05485 at 1151.299 kb on + strand, within BWI76_RS05485 at 1151.341 kb on - strand, within BWI76_RS05485 at 1151.518 kb on - strand at 1151.699 kb on + strand at 1151.707 kb on - strand at 1151.707 kb on - strand at 1151.707 kb on - strand at 1151.707 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.1 mg/ml remove 1,149,270 + +0.0 1,150,067 + BWI76_RS05480 0.16 +1.0 1,150,068 + BWI76_RS05480 0.16 -0.2 1,150,076 - BWI76_RS05480 0.17 +0.7 1,150,252 - BWI76_RS05480 0.40 +0.5 1,150,474 - BWI76_RS05480 0.69 -0.1 1,151,107 + BWI76_RS05485 0.58 +0.2 1,151,179 - BWI76_RS05485 0.67 -0.6 1,151,179 - BWI76_RS05485 0.67 +0.0 1,151,186 + BWI76_RS05485 0.68 +0.3 1,151,214 + BWI76_RS05485 0.72 +0.9 1,151,284 + BWI76_RS05485 0.82 -0.5 1,151,299 + BWI76_RS05485 0.84 +0.4 1,151,341 - BWI76_RS05485 0.89 -0.2 1,151,518 - +2.3 1,151,699 + -0.4 1,151,707 - -0.8 1,151,707 - -0.6 1,151,707 - +0.7 1,151,707 - -0.7
Or see this region's nucleotide sequence