Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05425

Experiment: A22 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05420 and BWI76_RS05425 are separated by 94 nucleotidesBWI76_RS05425 and BWI76_RS05430 are separated by 84 nucleotidesBWI76_RS05430 and BWI76_RS05435 are separated by 57 nucleotides BWI76_RS05420: BWI76_RS05420 - MFS transporter, at 1,138,012 to 1,139,202 _RS05420 BWI76_RS05425: BWI76_RS05425 - EEP domain-containing protein, at 1,139,297 to 1,140,103 _RS05425 BWI76_RS05430: BWI76_RS05430 - UbiE/COQ5 family methyltransferase, at 1,140,188 to 1,140,958 _RS05430 BWI76_RS05435: BWI76_RS05435 - murein transglycosylase D, at 1,141,016 to 1,142,383 _RS05435 Position (kb) 1139 1140 1141Strain fitness (log2 ratio) -2 -1 0 1at 1138.364 kb on + strand, within BWI76_RS05420at 1138.421 kb on + strand, within BWI76_RS05420at 1138.507 kb on - strand, within BWI76_RS05420at 1138.532 kb on + strand, within BWI76_RS05420at 1138.540 kb on - strand, within BWI76_RS05420at 1138.597 kb on + strand, within BWI76_RS05420at 1138.631 kb on + strand, within BWI76_RS05420at 1138.714 kb on - strand, within BWI76_RS05420at 1138.942 kb on - strand, within BWI76_RS05420at 1138.982 kb on - strand, within BWI76_RS05420at 1138.982 kb on - strand, within BWI76_RS05420at 1139.093 kb on - strandat 1139.205 kb on - strandat 1139.242 kb on + strandat 1139.367 kb on - strandat 1139.456 kb on + strand, within BWI76_RS05425at 1139.801 kb on - strand, within BWI76_RS05425at 1139.890 kb on - strand, within BWI76_RS05425at 1140.133 kb on + strandat 1140.211 kb on - strandat 1140.243 kb on + strandat 1140.251 kb on - strandat 1140.256 kb on - strandat 1140.481 kb on + strand, within BWI76_RS05430at 1140.540 kb on + strand, within BWI76_RS05430at 1140.752 kb on - strand, within BWI76_RS05430at 1141.076 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction A22 0.1 mg/ml
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1,138,364 + BWI76_RS05420 0.30 -0.7
1,138,421 + BWI76_RS05420 0.34 +0.3
1,138,507 - BWI76_RS05420 0.42 +0.2
1,138,532 + BWI76_RS05420 0.44 -0.2
1,138,540 - BWI76_RS05420 0.44 -0.2
1,138,597 + BWI76_RS05420 0.49 +0.2
1,138,631 + BWI76_RS05420 0.52 -0.4
1,138,714 - BWI76_RS05420 0.59 -0.3
1,138,942 - BWI76_RS05420 0.78 +0.3
1,138,982 - BWI76_RS05420 0.81 -0.0
1,138,982 - BWI76_RS05420 0.81 +1.4
1,139,093 - +0.4
1,139,205 - -1.3
1,139,242 + +1.5
1,139,367 - +0.3
1,139,456 + BWI76_RS05425 0.20 -0.8
1,139,801 - BWI76_RS05425 0.62 -2.3
1,139,890 - BWI76_RS05425 0.73 -0.7
1,140,133 + +0.4
1,140,211 - +0.7
1,140,243 + +0.8
1,140,251 - +0.0
1,140,256 - -0.2
1,140,481 + BWI76_RS05430 0.38 -0.3
1,140,540 + BWI76_RS05430 0.46 -0.9
1,140,752 - BWI76_RS05430 0.73 +0.2
1,141,076 - +0.3

Or see this region's nucleotide sequence