Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04775

Experiment: A22 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04765 and BWI76_RS04770 overlap by 8 nucleotidesBWI76_RS04770 and BWI76_RS04775 are separated by 1 nucleotidesBWI76_RS04775 and BWI76_RS04780 overlap by 4 nucleotides BWI76_RS04765: BWI76_RS04765 - UDP-N-acetylmuramate--L-alanine ligase, at 995,068 to 996,543 _RS04765 BWI76_RS04770: BWI76_RS04770 - D-alanine--D-alanine ligase, at 996,536 to 997,456 _RS04770 BWI76_RS04775: BWI76_RS04775 - cell division protein FtsQ, at 997,458 to 998,291 _RS04775 BWI76_RS04780: BWI76_RS04780 - cell division protein FtsA, at 998,288 to 999,544 _RS04780 Position (kb) 997 998 999Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 996.651 kb on + strand, within BWI76_RS04770at 997.255 kb on + strand, within BWI76_RS04770at 997.265 kb on + strand, within BWI76_RS04770at 997.351 kb on + strand, within BWI76_RS04770

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Per-strain Table

Position Strand Gene LocusTag Fraction A22 0.1 mg/ml
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996,651 + BWI76_RS04770 0.12 -4.2
997,255 + BWI76_RS04770 0.78 -1.6
997,265 + BWI76_RS04770 0.79 +0.1
997,351 + BWI76_RS04770 0.88 -1.7

Or see this region's nucleotide sequence