Experiment: A22 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04595 and BWI76_RS04600 are separated by 128 nucleotides BWI76_RS04600 and BWI76_RS04605 overlap by 14 nucleotides BWI76_RS04605 and BWI76_RS04610 overlap by 17 nucleotides
BWI76_RS04595: BWI76_RS04595 - DNA-binding transcriptional regulator AraC, at 956,778 to 957,623
_RS04595
BWI76_RS04600: BWI76_RS04600 - inner membrane protein, at 957,752 to 958,519
_RS04600
BWI76_RS04605: BWI76_RS04605 - thiamine ABC transporter ATP-binding protein, at 958,506 to 959,207
_RS04605
BWI76_RS04610: BWI76_RS04610 - thiamine/thiamine pyrophosphate ABC transporter permease ThiP, at 959,191 to 960,801
_RS04610
Position (kb) 957
958
959 Strain fitness (log2 ratio) -2
-1
0
1 at 956.908 kb on + strand, within BWI76_RS04595 at 956.916 kb on - strand, within BWI76_RS04595 at 956.921 kb on - strand, within BWI76_RS04595 at 956.966 kb on + strand, within BWI76_RS04595 at 957.063 kb on + strand, within BWI76_RS04595 at 957.197 kb on - strand, within BWI76_RS04595 at 957.217 kb on + strand, within BWI76_RS04595 at 957.225 kb on - strand, within BWI76_RS04595 at 957.302 kb on + strand, within BWI76_RS04595 at 957.487 kb on - strand, within BWI76_RS04595 at 957.491 kb on + strand, within BWI76_RS04595 at 957.710 kb on - strand at 957.711 kb on + strand at 957.882 kb on + strand, within BWI76_RS04600 at 957.910 kb on + strand, within BWI76_RS04600 at 958.400 kb on + strand, within BWI76_RS04600 at 958.852 kb on + strand, within BWI76_RS04605 at 959.409 kb on - strand, within BWI76_RS04610 at 959.490 kb on + strand, within BWI76_RS04610
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.1 mg/ml remove 956,908 + BWI76_RS04595 0.15 -1.7 956,916 - BWI76_RS04595 0.16 -0.3 956,921 - BWI76_RS04595 0.17 -0.2 956,966 + BWI76_RS04595 0.22 -0.1 957,063 + BWI76_RS04595 0.34 -0.5 957,197 - BWI76_RS04595 0.50 -2.2 957,217 + BWI76_RS04595 0.52 +0.3 957,225 - BWI76_RS04595 0.53 -0.7 957,302 + BWI76_RS04595 0.62 +0.2 957,487 - BWI76_RS04595 0.84 +0.1 957,491 + BWI76_RS04595 0.84 -0.5 957,710 - +0.5 957,711 + +0.5 957,882 + BWI76_RS04600 0.17 -0.8 957,910 + BWI76_RS04600 0.21 -0.9 958,400 + BWI76_RS04600 0.84 -0.4 958,852 + BWI76_RS04605 0.49 -0.1 959,409 - BWI76_RS04610 0.14 -0.6 959,490 + BWI76_RS04610 0.19 +0.1
Or see this region's nucleotide sequence