Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04530

Experiment: A22 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04525 and BWI76_RS04530 are separated by 112 nucleotidesBWI76_RS04530 and BWI76_RS04535 are separated by 53 nucleotides BWI76_RS04525: BWI76_RS04525 - 4-hydroxythreonine-4-phosphate dehydrogenase PdxA, at 935,442 to 936,431 _RS04525 BWI76_RS04530: BWI76_RS04530 - peptidylprolyl isomerase SurA, at 936,544 to 937,830 _RS04530 BWI76_RS04535: BWI76_RS04535 - LPS assembly protein LptD, at 937,884 to 940,226 _RS04535 Position (kb) 936 937 938Strain fitness (log2 ratio) -3 -2 -1 0 1at 935.946 kb on - strand, within BWI76_RS04525at 935.951 kb on - strand, within BWI76_RS04525at 935.997 kb on - strand, within BWI76_RS04525at 936.736 kb on + strand, within BWI76_RS04530at 936.830 kb on - strand, within BWI76_RS04530at 936.904 kb on - strand, within BWI76_RS04530at 937.353 kb on + strand, within BWI76_RS04530at 937.960 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction A22 0.1 mg/ml
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935,946 - BWI76_RS04525 0.51 -0.9
935,951 - BWI76_RS04525 0.51 -0.1
935,997 - BWI76_RS04525 0.56 +1.2
936,736 + BWI76_RS04530 0.15 -1.8
936,830 - BWI76_RS04530 0.22 -2.5
936,904 - BWI76_RS04530 0.28 -3.6
937,353 + BWI76_RS04530 0.63 -2.3
937,960 - +0.2

Or see this region's nucleotide sequence