Experiment: A22 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02665 and BWI76_RS02670 are separated by 4 nucleotides BWI76_RS02670 and BWI76_RS02675 are separated by 41 nucleotides BWI76_RS02675 and BWI76_RS02680 are separated by 146 nucleotides BWI76_RS02680 and BWI76_RS02685 are separated by 4 nucleotides BWI76_RS02685 and BWI76_RS02690 are separated by 139 nucleotides BWI76_RS02690 and BWI76_RS02695 overlap by 69 nucleotides
BWI76_RS02665: BWI76_RS02665 - primosomal replication protein N, at 542,874 to 543,188
_RS02665
BWI76_RS02670: BWI76_RS02670 - 30S ribosomal protein S18, at 543,193 to 543,420
_RS02670
BWI76_RS02675: BWI76_RS02675 - 50S ribosomal protein L9, at 543,462 to 543,911
_RS02675
BWI76_RS02680: BWI76_RS02680 - EamA family transporter, at 544,058 to 545,011
_RS02680
BWI76_RS02685: BWI76_RS02685 - opacity-associated protein, at 545,016 to 545,675
_RS02685
BWI76_RS02690: BWI76_RS02690 - hypothetical protein, at 545,815 to 546,012
_RS02690
BWI76_RS02695: BWI76_RS02695 - peptidyl-prolyl cis-trans isomerase, at 545,944 to 546,564
_RS02695
Position (kb)
544
545
546 Strain fitness (log2 ratio)
-2
-1
0
1 at 543.723 kb on + strand, within BWI76_RS02675 at 544.109 kb on + strand at 544.262 kb on + strand, within BWI76_RS02680 at 544.275 kb on - strand, within BWI76_RS02680 at 544.367 kb on - strand, within BWI76_RS02680 at 544.880 kb on + strand, within BWI76_RS02680 at 545.124 kb on + strand, within BWI76_RS02685 at 545.147 kb on + strand, within BWI76_RS02685 at 545.150 kb on - strand, within BWI76_RS02685 at 545.207 kb on + strand, within BWI76_RS02685 at 545.224 kb on + strand, within BWI76_RS02685 at 545.265 kb on - strand, within BWI76_RS02685 at 545.499 kb on + strand, within BWI76_RS02685 at 545.507 kb on - strand, within BWI76_RS02685 at 545.713 kb on + strand at 545.776 kb on - strand at 545.776 kb on - strand at 545.782 kb on + strand at 545.867 kb on + strand, within BWI76_RS02690 at 545.966 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.1 mg/ml remove 543,723 + BWI76_RS02675 0.58 -1.2 544,109 + +0.4 544,262 + BWI76_RS02680 0.21 +0.7 544,275 - BWI76_RS02680 0.23 -0.8 544,367 - BWI76_RS02680 0.32 -1.3 544,880 + BWI76_RS02680 0.86 -2.1 545,124 + BWI76_RS02685 0.16 +0.8 545,147 + BWI76_RS02685 0.20 +0.7 545,150 - BWI76_RS02685 0.20 -0.3 545,207 + BWI76_RS02685 0.29 +0.1 545,224 + BWI76_RS02685 0.32 -0.2 545,265 - BWI76_RS02685 0.38 -2.4 545,499 + BWI76_RS02685 0.73 -0.1 545,507 - BWI76_RS02685 0.74 -0.1 545,713 + -0.6 545,776 - -0.1 545,776 - +0.9 545,782 + -0.0 545,867 + BWI76_RS02690 0.26 +0.1 545,966 + -0.4
Or see this region's nucleotide sequence