Experiment: A22 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02530 and BWI76_RS02535 are separated by 92 nucleotides BWI76_RS02535 and BWI76_RS02540 are separated by 75 nucleotides BWI76_RS02540 and BWI76_RS02545 are separated by 65 nucleotides
BWI76_RS02530: BWI76_RS02530 - tRNA dimethylallyltransferase, at 518,914 to 519,864
_RS02530
BWI76_RS02535: BWI76_RS02535 - RNA-binding protein Hfq, at 519,957 to 520,265
_RS02535
BWI76_RS02540: BWI76_RS02540 - GTPase HflX, at 520,341 to 521,621
_RS02540
BWI76_RS02545: BWI76_RS02545 - protease modulator HflK, at 521,687 to 522,943
_RS02545
Position (kb) 520
521
522 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 519.530 kb on + strand, within BWI76_RS02530 at 519.776 kb on + strand at 519.853 kb on + strand at 519.882 kb on + strand at 519.922 kb on + strand at 520.239 kb on + strand at 520.333 kb on + strand at 520.365 kb on - strand at 520.427 kb on - strand at 520.528 kb on - strand, within BWI76_RS02540 at 520.794 kb on - strand, within BWI76_RS02540 at 520.799 kb on - strand, within BWI76_RS02540 at 520.825 kb on - strand, within BWI76_RS02540 at 520.849 kb on + strand, within BWI76_RS02540 at 521.003 kb on + strand, within BWI76_RS02540 at 521.011 kb on - strand, within BWI76_RS02540 at 521.252 kb on + strand, within BWI76_RS02540 at 521.311 kb on + strand, within BWI76_RS02540 at 521.357 kb on + strand, within BWI76_RS02540 at 521.441 kb on + strand, within BWI76_RS02540 at 521.516 kb on - strand at 521.541 kb on + strand at 521.550 kb on - strand at 521.684 kb on + strand at 522.172 kb on - strand, within BWI76_RS02545 at 522.186 kb on - strand, within BWI76_RS02545 at 522.375 kb on + strand, within BWI76_RS02545 at 522.375 kb on + strand, within BWI76_RS02545 at 522.375 kb on + strand, within BWI76_RS02545 at 522.383 kb on - strand, within BWI76_RS02545 at 522.576 kb on - strand, within BWI76_RS02545 at 522.592 kb on + strand, within BWI76_RS02545
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.1 mg/ml remove 519,530 + BWI76_RS02530 0.65 -1.7 519,776 + -3.3 519,853 + -1.1 519,882 + -0.2 519,922 + -1.2 520,239 + -4.5 520,333 + -1.7 520,365 - +0.2 520,427 - -0.6 520,528 - BWI76_RS02540 0.15 -0.9 520,794 - BWI76_RS02540 0.35 -1.2 520,799 - BWI76_RS02540 0.36 -1.5 520,825 - BWI76_RS02540 0.38 -1.0 520,849 + BWI76_RS02540 0.40 -0.1 521,003 + BWI76_RS02540 0.52 -0.4 521,011 - BWI76_RS02540 0.52 -1.6 521,252 + BWI76_RS02540 0.71 -0.5 521,311 + BWI76_RS02540 0.76 -0.3 521,357 + BWI76_RS02540 0.79 -0.9 521,441 + BWI76_RS02540 0.86 -0.7 521,516 - -1.0 521,541 + -0.4 521,550 - +0.5 521,684 + -0.9 522,172 - BWI76_RS02545 0.39 -0.7 522,186 - BWI76_RS02545 0.40 -1.1 522,375 + BWI76_RS02545 0.55 -0.9 522,375 + BWI76_RS02545 0.55 -0.0 522,375 + BWI76_RS02545 0.55 -1.1 522,383 - BWI76_RS02545 0.55 -0.4 522,576 - BWI76_RS02545 0.71 -0.5 522,592 + BWI76_RS02545 0.72 -0.6
Or see this region's nucleotide sequence