Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01325

Experiment: A22 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01315 and BWI76_RS01320 are separated by 76 nucleotidesBWI76_RS01320 and BWI76_RS01325 are separated by 3 nucleotidesBWI76_RS01325 and BWI76_RS01330 are separated by 2 nucleotidesBWI76_RS01330 and BWI76_RS01335 are separated by 42 nucleotides BWI76_RS01315: BWI76_RS01315 - ubiquinone biosynthesis regulatory protein kinase UbiB, at 269,971 to 271,611 _RS01315 BWI76_RS01320: BWI76_RS01320 - twin-arginine translocase subunit TatA, at 271,688 to 271,939 _RS01320 BWI76_RS01325: BWI76_RS01325 - twin-arginine translocase subunit TatB, at 271,943 to 272,482 _RS01325 BWI76_RS01330: BWI76_RS01330 - twin-arginine translocase subunit TatC, at 272,485 to 273,258 _RS01330 BWI76_RS01335: BWI76_RS01335 - DNase TatD, at 273,301 to 274,083 _RS01335 Position (kb) 271 272 273Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 271.595 kb on + strandat 271.692 kb on - strandat 271.933 kb on + strandat 272.135 kb on - strand, within BWI76_RS01325at 272.158 kb on - strand, within BWI76_RS01325at 272.347 kb on - strand, within BWI76_RS01325at 272.522 kb on + strandat 272.781 kb on + strand, within BWI76_RS01330at 272.796 kb on - strand, within BWI76_RS01330at 272.822 kb on + strand, within BWI76_RS01330at 272.835 kb on + strand, within BWI76_RS01330at 272.915 kb on + strand, within BWI76_RS01330at 273.194 kb on - strandat 273.210 kb on - strandat 273.253 kb on + strandat 273.358 kb on + strandat 273.407 kb on + strand, within BWI76_RS01335at 273.479 kb on + strand, within BWI76_RS01335

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Per-strain Table

Position Strand Gene LocusTag Fraction A22 0.1 mg/ml
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271,595 + +0.2
271,692 - -1.7
271,933 + -1.1
272,135 - BWI76_RS01325 0.36 -1.0
272,158 - BWI76_RS01325 0.40 -0.7
272,347 - BWI76_RS01325 0.75 -1.7
272,522 + -0.7
272,781 + BWI76_RS01330 0.38 -1.5
272,796 - BWI76_RS01330 0.40 -0.5
272,822 + BWI76_RS01330 0.44 -1.1
272,835 + BWI76_RS01330 0.45 -1.4
272,915 + BWI76_RS01330 0.56 -1.3
273,194 - -4.4
273,210 - -0.7
273,253 + -0.3
273,358 + -0.2
273,407 + BWI76_RS01335 0.14 -0.6
273,479 + BWI76_RS01335 0.23 -0.9

Or see this region's nucleotide sequence