Experiment: A22 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01315 and BWI76_RS01320 are separated by 76 nucleotides BWI76_RS01320 and BWI76_RS01325 are separated by 3 nucleotides BWI76_RS01325 and BWI76_RS01330 are separated by 2 nucleotides BWI76_RS01330 and BWI76_RS01335 are separated by 42 nucleotides
BWI76_RS01315: BWI76_RS01315 - ubiquinone biosynthesis regulatory protein kinase UbiB, at 269,971 to 271,611
_RS01315
BWI76_RS01320: BWI76_RS01320 - twin-arginine translocase subunit TatA, at 271,688 to 271,939
_RS01320
BWI76_RS01325: BWI76_RS01325 - twin-arginine translocase subunit TatB, at 271,943 to 272,482
_RS01325
BWI76_RS01330: BWI76_RS01330 - twin-arginine translocase subunit TatC, at 272,485 to 273,258
_RS01330
BWI76_RS01335: BWI76_RS01335 - DNase TatD, at 273,301 to 274,083
_RS01335
Position (kb) 271
272
273 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 271.595 kb on + strand at 271.692 kb on - strand at 271.933 kb on + strand at 272.135 kb on - strand, within BWI76_RS01325 at 272.158 kb on - strand, within BWI76_RS01325 at 272.347 kb on - strand, within BWI76_RS01325 at 272.522 kb on + strand at 272.781 kb on + strand, within BWI76_RS01330 at 272.796 kb on - strand, within BWI76_RS01330 at 272.822 kb on + strand, within BWI76_RS01330 at 272.835 kb on + strand, within BWI76_RS01330 at 272.915 kb on + strand, within BWI76_RS01330 at 273.194 kb on - strand at 273.210 kb on - strand at 273.253 kb on + strand at 273.358 kb on + strand at 273.407 kb on + strand, within BWI76_RS01335 at 273.479 kb on + strand, within BWI76_RS01335
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.1 mg/ml remove 271,595 + +0.2 271,692 - -1.7 271,933 + -1.1 272,135 - BWI76_RS01325 0.36 -1.0 272,158 - BWI76_RS01325 0.40 -0.7 272,347 - BWI76_RS01325 0.75 -1.7 272,522 + -0.7 272,781 + BWI76_RS01330 0.38 -1.5 272,796 - BWI76_RS01330 0.40 -0.5 272,822 + BWI76_RS01330 0.44 -1.1 272,835 + BWI76_RS01330 0.45 -1.4 272,915 + BWI76_RS01330 0.56 -1.3 273,194 - -4.4 273,210 - -0.7 273,253 + -0.3 273,358 + -0.2 273,407 + BWI76_RS01335 0.14 -0.6 273,479 + BWI76_RS01335 0.23 -0.9
Or see this region's nucleotide sequence