Experiment: A22 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01210 and BWI76_RS01215 overlap by 1 nucleotides BWI76_RS01215 and BWI76_RS01220 are separated by 77 nucleotides BWI76_RS01220 and BWI76_RS01225 are separated by 354 nucleotides
BWI76_RS01210: BWI76_RS01210 - tyrosine recombinase XerC, at 246,885 to 247,787
_RS01210
BWI76_RS01215: BWI76_RS01215 - flavin mononucleotide phosphatase, at 247,787 to 248,503
_RS01215
BWI76_RS01220: BWI76_RS01220 - DNA-dependent helicase II, at 248,581 to 250,743
_RS01220
BWI76_RS01225: BWI76_RS01225 - magnesium transporter CorA, at 251,098 to 252,048
_RS01225
Position (kb) 248
249
250
251 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 247.835 kb on - strand at 247.857 kb on + strand at 247.916 kb on - strand, within BWI76_RS01215 at 248.806 kb on - strand, within BWI76_RS01220 at 248.808 kb on + strand, within BWI76_RS01220 at 248.811 kb on - strand, within BWI76_RS01220 at 248.824 kb on - strand, within BWI76_RS01220 at 248.878 kb on + strand, within BWI76_RS01220 at 249.087 kb on - strand, within BWI76_RS01220 at 249.369 kb on - strand, within BWI76_RS01220 at 249.617 kb on + strand, within BWI76_RS01220 at 249.695 kb on - strand, within BWI76_RS01220 at 249.796 kb on + strand, within BWI76_RS01220 at 249.853 kb on - strand, within BWI76_RS01220 at 250.211 kb on - strand, within BWI76_RS01220 at 250.245 kb on + strand, within BWI76_RS01220 at 250.399 kb on + strand, within BWI76_RS01220 at 250.507 kb on - strand, within BWI76_RS01220 at 250.979 kb on + strand at 251.012 kb on + strand at 251.018 kb on + strand at 251.188 kb on - strand at 251.236 kb on + strand, within BWI76_RS01225 at 251.255 kb on + strand, within BWI76_RS01225 at 251.298 kb on + strand, within BWI76_RS01225 at 251.366 kb on + strand, within BWI76_RS01225 at 251.414 kb on + strand, within BWI76_RS01225 at 251.547 kb on + strand, within BWI76_RS01225
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.1 mg/ml remove 247,835 - +0.2 247,857 + +0.5 247,916 - BWI76_RS01215 0.18 -0.5 248,806 - BWI76_RS01220 0.10 -3.4 248,808 + BWI76_RS01220 0.10 -1.4 248,811 - BWI76_RS01220 0.11 -2.6 248,824 - BWI76_RS01220 0.11 -2.4 248,878 + BWI76_RS01220 0.14 -1.8 249,087 - BWI76_RS01220 0.23 -1.7 249,369 - BWI76_RS01220 0.36 -3.5 249,617 + BWI76_RS01220 0.48 -2.6 249,695 - BWI76_RS01220 0.52 -0.8 249,796 + BWI76_RS01220 0.56 -3.7 249,853 - BWI76_RS01220 0.59 -0.2 250,211 - BWI76_RS01220 0.75 -1.8 250,245 + BWI76_RS01220 0.77 -1.3 250,399 + BWI76_RS01220 0.84 -0.4 250,507 - BWI76_RS01220 0.89 -0.1 250,979 + +1.4 251,012 + -0.2 251,018 + +0.2 251,188 - +0.4 251,236 + BWI76_RS01225 0.15 +0.4 251,255 + BWI76_RS01225 0.17 +0.3 251,298 + BWI76_RS01225 0.21 -0.8 251,366 + BWI76_RS01225 0.28 +0.6 251,414 + BWI76_RS01225 0.33 -0.5 251,547 + BWI76_RS01225 0.47 +0.8
Or see this region's nucleotide sequence