Experiment: A22 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01120 and BWI76_RS01125 overlap by 4 nucleotides BWI76_RS01125 and BWI76_RS01130 overlap by 4 nucleotides
BWI76_RS01120: BWI76_RS01120 - O-antigen translocase, at 230,576 to 231,826
_RS01120
BWI76_RS01125: BWI76_RS01125 - 4-alpha-L-fucosyltransferase, at 231,823 to 232,902
_RS01125
BWI76_RS01130: BWI76_RS01130 - enterobacterial common antigen polymerase, at 232,899 to 234,245
_RS01130
Position (kb) 231
232
233 Strain fitness (log2 ratio) -2
-1
0
1 at 230.926 kb on - strand, within BWI76_RS01120 at 230.953 kb on + strand, within BWI76_RS01120 at 230.953 kb on + strand, within BWI76_RS01120 at 231.208 kb on + strand, within BWI76_RS01120 at 231.388 kb on + strand, within BWI76_RS01120 at 231.410 kb on + strand, within BWI76_RS01120 at 231.627 kb on - strand, within BWI76_RS01120 at 231.640 kb on + strand, within BWI76_RS01120 at 231.739 kb on - strand at 231.759 kb on - strand at 231.759 kb on - strand at 231.787 kb on + strand at 231.795 kb on - strand at 231.824 kb on + strand at 231.832 kb on - strand at 231.877 kb on + strand at 231.898 kb on + strand at 232.139 kb on - strand, within BWI76_RS01125 at 232.219 kb on - strand, within BWI76_RS01125 at 232.534 kb on + strand, within BWI76_RS01125 at 232.534 kb on + strand, within BWI76_RS01125 at 232.534 kb on + strand, within BWI76_RS01125 at 232.542 kb on - strand, within BWI76_RS01125 at 232.712 kb on - strand, within BWI76_RS01125 at 232.727 kb on + strand, within BWI76_RS01125 at 232.768 kb on + strand, within BWI76_RS01125 at 232.795 kb on - strand at 232.839 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.1 mg/ml remove 230,926 - BWI76_RS01120 0.28 -1.0 230,953 + BWI76_RS01120 0.30 -0.6 230,953 + BWI76_RS01120 0.30 -0.4 231,208 + BWI76_RS01120 0.51 -0.8 231,388 + BWI76_RS01120 0.65 -1.0 231,410 + BWI76_RS01120 0.67 -0.0 231,627 - BWI76_RS01120 0.84 -1.8 231,640 + BWI76_RS01120 0.85 +0.2 231,739 - -0.6 231,759 - -2.0 231,759 - -1.1 231,787 + -0.2 231,795 - -0.2 231,824 + -0.4 231,832 - -0.1 231,877 + -0.2 231,898 + -1.5 232,139 - BWI76_RS01125 0.29 -1.6 232,219 - BWI76_RS01125 0.37 -2.4 232,534 + BWI76_RS01125 0.66 -2.5 232,534 + BWI76_RS01125 0.66 -2.6 232,534 + BWI76_RS01125 0.66 -1.0 232,542 - BWI76_RS01125 0.67 -1.4 232,712 - BWI76_RS01125 0.82 -0.1 232,727 + BWI76_RS01125 0.84 +0.1 232,768 + BWI76_RS01125 0.88 -0.4 232,795 - -0.6 232,839 - -1.0
Or see this region's nucleotide sequence