Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01115

Experiment: A22 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01105 and BWI76_RS01110 overlap by 23 nucleotidesBWI76_RS01110 and BWI76_RS01115 are separated by 4 nucleotidesBWI76_RS01115 and BWI76_RS01120 are separated by 1 nucleotides BWI76_RS01105: BWI76_RS01105 - glucose-1-phosphate thymidylyltransferase, at 227,906 to 228,787 _RS01105 BWI76_RS01110: BWI76_RS01110 - TDP-fucosamine acetyltransferase, at 228,765 to 229,439 _RS01110 BWI76_RS01115: BWI76_RS01115 - dTDP-4-amino-4,6-dideoxygalactose transaminase, at 229,444 to 230,574 _RS01115 BWI76_RS01120: BWI76_RS01120 - O-antigen translocase, at 230,576 to 231,826 _RS01120 Position (kb) 229 230 231Strain fitness (log2 ratio) -3 -2 -1 0 1at 228.654 kb on + strand, within BWI76_RS01105at 228.789 kb on - strandat 228.839 kb on - strand, within BWI76_RS01110at 228.927 kb on + strand, within BWI76_RS01110at 229.276 kb on - strand, within BWI76_RS01110at 229.279 kb on + strand, within BWI76_RS01110at 229.315 kb on + strand, within BWI76_RS01110at 229.536 kb on - strandat 229.602 kb on + strand, within BWI76_RS01115at 229.711 kb on - strand, within BWI76_RS01115at 229.716 kb on - strand, within BWI76_RS01115at 230.473 kb on + strandat 230.481 kb on - strandat 230.528 kb on - strandat 230.592 kb on - strandat 230.926 kb on - strand, within BWI76_RS01120at 230.953 kb on + strand, within BWI76_RS01120at 230.953 kb on + strand, within BWI76_RS01120at 231.208 kb on + strand, within BWI76_RS01120at 231.388 kb on + strand, within BWI76_RS01120at 231.410 kb on + strand, within BWI76_RS01120

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Per-strain Table

Position Strand Gene LocusTag Fraction A22 0.1 mg/ml
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228,654 + BWI76_RS01105 0.85 -0.5
228,789 - -1.4
228,839 - BWI76_RS01110 0.11 -1.2
228,927 + BWI76_RS01110 0.24 -1.0
229,276 - BWI76_RS01110 0.76 -0.3
229,279 + BWI76_RS01110 0.76 -0.8
229,315 + BWI76_RS01110 0.81 -1.5
229,536 - -1.9
229,602 + BWI76_RS01115 0.14 -0.7
229,711 - BWI76_RS01115 0.24 -0.7
229,716 - BWI76_RS01115 0.24 -1.2
230,473 + -0.5
230,481 - +0.2
230,528 - -3.4
230,592 - -1.2
230,926 - BWI76_RS01120 0.28 -1.0
230,953 + BWI76_RS01120 0.30 -0.6
230,953 + BWI76_RS01120 0.30 -0.4
231,208 + BWI76_RS01120 0.51 -0.8
231,388 + BWI76_RS01120 0.65 -1.0
231,410 + BWI76_RS01120 0.67 -0.0

Or see this region's nucleotide sequence