Experiment: A22 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01090 and BWI76_RS01095 overlap by 4 nucleotides BWI76_RS01095 and BWI76_RS01100 overlap by 4 nucleotides
BWI76_RS01090: BWI76_RS01090 - UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing), at 224,433 to 225,563
_RS01090
BWI76_RS01095: BWI76_RS01095 - UDP-N-acetyl-D-mannosamine dehydrogenase, at 225,560 to 226,822
_RS01095
BWI76_RS01100: BWI76_RS01100 - dTDP-glucose 4,6-dehydratase, at 226,819 to 227,887
_RS01100
Position (kb) 225
226
227 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 224.637 kb on - strand, within BWI76_RS01090 at 224.826 kb on - strand, within BWI76_RS01090 at 224.859 kb on + strand, within BWI76_RS01090 at 225.000 kb on - strand, within BWI76_RS01090 at 225.010 kb on + strand, within BWI76_RS01090 at 225.020 kb on + strand, within BWI76_RS01090 at 225.502 kb on + strand at 225.589 kb on + strand at 226.034 kb on - strand, within BWI76_RS01095 at 226.533 kb on + strand, within BWI76_RS01095 at 226.541 kb on - strand, within BWI76_RS01095 at 226.711 kb on + strand at 226.776 kb on + strand at 226.901 kb on + strand at 226.918 kb on + strand at 226.979 kb on + strand, within BWI76_RS01100 at 227.132 kb on + strand, within BWI76_RS01100 at 227.132 kb on + strand, within BWI76_RS01100 at 227.132 kb on + strand, within BWI76_RS01100 at 227.140 kb on - strand, within BWI76_RS01100 at 227.142 kb on + strand, within BWI76_RS01100 at 227.227 kb on + strand, within BWI76_RS01100 at 227.235 kb on - strand, within BWI76_RS01100 at 227.642 kb on - strand, within BWI76_RS01100
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.1 mg/ml remove 224,637 - BWI76_RS01090 0.18 +0.4 224,826 - BWI76_RS01090 0.35 +0.2 224,859 + BWI76_RS01090 0.38 -0.5 225,000 - BWI76_RS01090 0.50 -0.1 225,010 + BWI76_RS01090 0.51 +0.1 225,020 + BWI76_RS01090 0.52 -0.0 225,502 + +0.0 225,589 + -2.5 226,034 - BWI76_RS01095 0.38 -2.8 226,533 + BWI76_RS01095 0.77 -0.5 226,541 - BWI76_RS01095 0.78 -0.4 226,711 + -1.0 226,776 + -0.8 226,901 + -0.5 226,918 + +0.1 226,979 + BWI76_RS01100 0.15 -0.9 227,132 + BWI76_RS01100 0.29 -0.4 227,132 + BWI76_RS01100 0.29 +0.9 227,132 + BWI76_RS01100 0.29 -0.6 227,140 - BWI76_RS01100 0.30 -1.6 227,142 + BWI76_RS01100 0.30 +0.1 227,227 + BWI76_RS01100 0.38 +0.3 227,235 - BWI76_RS01100 0.39 +0.3 227,642 - BWI76_RS01100 0.77 -0.7
Or see this region's nucleotide sequence