Experiment: A22 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01075 and BWI76_RS01080 are separated by 242 nucleotides BWI76_RS01080 and BWI76_RS01085 are separated by 21 nucleotides
BWI76_RS01075: BWI76_RS01075 - transcription termination factor Rho, at 220,712 to 221,971
_RS01075
BWI76_RS01080: BWI76_RS01080 - undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase, at 222,214 to 223,308
_RS01080
BWI76_RS01085: BWI76_RS01085 - polysaccharide chain length modulation protein, at 223,330 to 224,376
_RS01085
Position (kb) 222
223
224 Strain fitness (log2 ratio) -2
-1
0
1 at 222.063 kb on + strand at 222.063 kb on + strand at 222.071 kb on - strand at 222.071 kb on - strand at 222.073 kb on - strand at 222.081 kb on - strand at 222.081 kb on - strand at 222.137 kb on + strand at 222.142 kb on + strand at 222.145 kb on - strand at 222.157 kb on + strand at 222.159 kb on - strand at 222.171 kb on + strand at 222.171 kb on + strand at 222.179 kb on - strand at 222.179 kb on - strand at 222.179 kb on - strand at 222.179 kb on - strand at 222.179 kb on - strand at 222.179 kb on - strand at 222.196 kb on + strand at 222.196 kb on + strand at 222.201 kb on + strand at 222.203 kb on + strand at 222.224 kb on - strand at 222.270 kb on + strand at 222.310 kb on - strand at 222.310 kb on - strand at 222.345 kb on - strand, within BWI76_RS01080 at 222.353 kb on - strand, within BWI76_RS01080 at 222.434 kb on + strand, within BWI76_RS01080 at 222.435 kb on + strand, within BWI76_RS01080 at 222.439 kb on + strand, within BWI76_RS01080 at 222.441 kb on - strand, within BWI76_RS01080 at 222.441 kb on - strand, within BWI76_RS01080 at 222.447 kb on - strand, within BWI76_RS01080 at 222.447 kb on - strand, within BWI76_RS01080 at 222.449 kb on + strand, within BWI76_RS01080 at 222.496 kb on - strand, within BWI76_RS01080 at 222.612 kb on - strand, within BWI76_RS01080 at 222.629 kb on + strand, within BWI76_RS01080 at 222.647 kb on - strand, within BWI76_RS01080 at 222.703 kb on + strand, within BWI76_RS01080 at 222.762 kb on - strand, within BWI76_RS01080 at 222.842 kb on + strand, within BWI76_RS01080 at 222.921 kb on + strand, within BWI76_RS01080 at 222.935 kb on + strand, within BWI76_RS01080 at 223.009 kb on - strand, within BWI76_RS01080 at 223.086 kb on + strand, within BWI76_RS01080 at 223.116 kb on - strand, within BWI76_RS01080 at 223.133 kb on + strand, within BWI76_RS01080 at 223.238 kb on + strand at 223.261 kb on - strand at 223.301 kb on - strand at 223.325 kb on - strand at 223.326 kb on + strand at 223.334 kb on - strand at 223.419 kb on + strand at 223.438 kb on + strand, within BWI76_RS01085 at 223.608 kb on + strand, within BWI76_RS01085 at 223.697 kb on + strand, within BWI76_RS01085 at 223.917 kb on + strand, within BWI76_RS01085 at 224.006 kb on + strand, within BWI76_RS01085 at 224.069 kb on + strand, within BWI76_RS01085 at 224.097 kb on + strand, within BWI76_RS01085 at 224.119 kb on - strand, within BWI76_RS01085 at 224.184 kb on - strand, within BWI76_RS01085
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.1 mg/ml remove 222,063 + -1.4 222,063 + +1.3 222,071 - +0.0 222,071 - +0.2 222,073 - +1.1 222,081 - +0.2 222,081 - +0.5 222,137 + +0.9 222,142 + -0.6 222,145 - -0.4 222,157 + +0.0 222,159 - +0.3 222,171 + -1.3 222,171 + -1.1 222,179 - -0.4 222,179 - +0.0 222,179 - -0.5 222,179 - -0.3 222,179 - -0.4 222,179 - -2.6 222,196 + -0.4 222,196 + -0.3 222,201 + -0.5 222,203 + -1.2 222,224 - -1.1 222,270 + -1.4 222,310 - -1.5 222,310 - +0.7 222,345 - BWI76_RS01080 0.12 +0.2 222,353 - BWI76_RS01080 0.13 -0.4 222,434 + BWI76_RS01080 0.20 -1.9 222,435 + BWI76_RS01080 0.20 +0.9 222,439 + BWI76_RS01080 0.21 -1.6 222,441 - BWI76_RS01080 0.21 +0.4 222,441 - BWI76_RS01080 0.21 -1.0 222,447 - BWI76_RS01080 0.21 -1.2 222,447 - BWI76_RS01080 0.21 -1.0 222,449 + BWI76_RS01080 0.21 -1.5 222,496 - BWI76_RS01080 0.26 -0.2 222,612 - BWI76_RS01080 0.36 +1.4 222,629 + BWI76_RS01080 0.38 +0.3 222,647 - BWI76_RS01080 0.40 -0.6 222,703 + BWI76_RS01080 0.45 -1.1 222,762 - BWI76_RS01080 0.50 -0.6 222,842 + BWI76_RS01080 0.57 -1.5 222,921 + BWI76_RS01080 0.65 -0.1 222,935 + BWI76_RS01080 0.66 -1.7 223,009 - BWI76_RS01080 0.73 -0.4 223,086 + BWI76_RS01080 0.80 -2.5 223,116 - BWI76_RS01080 0.82 +1.0 223,133 + BWI76_RS01080 0.84 -0.5 223,238 + -0.5 223,261 - -0.9 223,301 - -1.4 223,325 - -0.9 223,326 + -0.2 223,334 - -0.7 223,419 + +0.0 223,438 + BWI76_RS01085 0.10 -0.1 223,608 + BWI76_RS01085 0.27 +0.1 223,697 + BWI76_RS01085 0.35 -0.6 223,917 + BWI76_RS01085 0.56 +0.3 224,006 + BWI76_RS01085 0.65 -0.1 224,069 + BWI76_RS01085 0.71 +0.1 224,097 + BWI76_RS01085 0.73 +0.7 224,119 - BWI76_RS01085 0.75 -0.4 224,184 - BWI76_RS01085 0.82 -1.0
Or see this region's nucleotide sequence