Experiment: Cephalothin 0.16 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26390 and BWI76_RS26395 overlap by 8 nucleotides BWI76_RS26395 and BWI76_RS26400 are separated by 2 nucleotides BWI76_RS26400 and BWI76_RS26405 are separated by 192 nucleotides BWI76_RS26405 and BWI76_RS26410 overlap by 11 nucleotides
BWI76_RS26390: BWI76_RS26390 - cell division protein FtsX, at 5,463,317 to 5,464,375
_RS26390
BWI76_RS26395: BWI76_RS26395 - cell division protein FtsE, at 5,464,368 to 5,465,036
_RS26395
BWI76_RS26400: BWI76_RS26400 - signal recognition particle-docking protein FtsY, at 5,465,039 to 5,466,553
_RS26400
BWI76_RS26405: BWI76_RS26405 - 16S rRNA (guanine(966)-N(2))-methyltransferase, at 5,466,746 to 5,467,342
_RS26405
BWI76_RS26410: BWI76_RS26410 - membrane protein, at 5,467,332 to 5,467,610
_RS26410
Position (kb) 5465
5466
5467 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5464.056 kb on + strand, within BWI76_RS26390 at 5464.491 kb on - strand, within BWI76_RS26395 at 5464.491 kb on - strand, within BWI76_RS26395 at 5464.767 kb on - strand, within BWI76_RS26395 at 5466.588 kb on - strand at 5466.676 kb on + strand at 5466.953 kb on - strand, within BWI76_RS26405 at 5467.051 kb on + strand, within BWI76_RS26405 at 5467.305 kb on + strand at 5467.437 kb on + strand, within BWI76_RS26410 at 5467.473 kb on - strand, within BWI76_RS26410
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.16 mg/ml remove 5,464,056 + BWI76_RS26390 0.70 -2.2 5,464,491 - BWI76_RS26395 0.18 -2.5 5,464,491 - BWI76_RS26395 0.18 -2.4 5,464,767 - BWI76_RS26395 0.60 -2.8 5,466,588 - +0.0 5,466,676 + -0.1 5,466,953 - BWI76_RS26405 0.35 +1.1 5,467,051 + BWI76_RS26405 0.51 -0.2 5,467,305 + +0.0 5,467,437 + BWI76_RS26410 0.38 -0.4 5,467,473 - BWI76_RS26410 0.51 +0.1
Or see this region's nucleotide sequence