Experiment: Cephalothin 0.16 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20055 and BWI76_RS20060 overlap by 4 nucleotides BWI76_RS20060 and BWI76_RS20065 overlap by 1 nucleotides BWI76_RS20065 and BWI76_RS20070 are separated by 14 nucleotides BWI76_RS20070 and BWI76_RS20075 overlap by 4 nucleotides
BWI76_RS20055: BWI76_RS20055 - 2-succinylbenzoate-CoA ligase, at 4,201,216 to 4,202,607
_RS20055
BWI76_RS20060: BWI76_RS20060 - o-succinylbenzoate synthase, at 4,202,604 to 4,203,569
_RS20060
BWI76_RS20065: BWI76_RS20065 - 1,4-dihydroxy-2-naphthoyl-CoA synthase, at 4,203,569 to 4,204,426
_RS20065
BWI76_RS20070: BWI76_RS20070 - 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase, at 4,204,441 to 4,205,199
_RS20070
BWI76_RS20075: BWI76_RS20075 - 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylic-acid synthase, at 4,205,196 to 4,206,866
_RS20075
Position (kb) 4203
4204
4205 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4202.602 kb on + strand at 4202.610 kb on - strand at 4202.632 kb on - strand at 4202.687 kb on + strand at 4202.728 kb on - strand, within BWI76_RS20060 at 4202.798 kb on + strand, within BWI76_RS20060 at 4202.935 kb on - strand, within BWI76_RS20060 at 4203.063 kb on - strand, within BWI76_RS20060 at 4203.151 kb on + strand, within BWI76_RS20060 at 4203.174 kb on - strand, within BWI76_RS20060 at 4203.306 kb on - strand, within BWI76_RS20060 at 4203.763 kb on - strand, within BWI76_RS20065 at 4203.827 kb on - strand, within BWI76_RS20065 at 4203.913 kb on + strand, within BWI76_RS20065 at 4204.105 kb on + strand, within BWI76_RS20065 at 4204.489 kb on + strand at 4204.777 kb on + strand, within BWI76_RS20070 at 4205.061 kb on + strand, within BWI76_RS20070 at 4205.182 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.16 mg/ml remove 4,202,602 + +0.2 4,202,610 - +0.1 4,202,632 - -0.1 4,202,687 + +0.1 4,202,728 - BWI76_RS20060 0.13 -0.1 4,202,798 + BWI76_RS20060 0.20 -1.8 4,202,935 - BWI76_RS20060 0.34 -0.7 4,203,063 - BWI76_RS20060 0.48 -0.2 4,203,151 + BWI76_RS20060 0.57 +1.0 4,203,174 - BWI76_RS20060 0.59 +0.2 4,203,306 - BWI76_RS20060 0.73 -0.0 4,203,763 - BWI76_RS20065 0.23 -1.2 4,203,827 - BWI76_RS20065 0.30 -0.2 4,203,913 + BWI76_RS20065 0.40 -0.2 4,204,105 + BWI76_RS20065 0.62 -2.0 4,204,489 + -0.0 4,204,777 + BWI76_RS20070 0.44 +2.6 4,205,061 + BWI76_RS20070 0.82 +1.3 4,205,182 - +0.2
Or see this region's nucleotide sequence