Experiment: Cephalothin 0.16 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18305 and BWI76_RS18310 are separated by 190 nucleotides BWI76_RS18310 and BWI76_RS18315 are separated by 13 nucleotides BWI76_RS18315 and BWI76_RS18320 are separated by 38 nucleotides BWI76_RS18320 and BWI76_RS18325 are separated by 151 nucleotides BWI76_RS18325 and BWI76_RS18330 are separated by 57 nucleotides
BWI76_RS18305: BWI76_RS18305 - dihydrodipicolinate synthase family protein, at 3,803,453 to 3,804,340
_RS18305
BWI76_RS18310: BWI76_RS18310 - tRNA-Leu, at 3,804,531 to 3,804,617
_RS18310
BWI76_RS18315: BWI76_RS18315 - tRNA-Cys, at 3,804,631 to 3,804,704
_RS18315
BWI76_RS18320: BWI76_RS18320 - tRNA-Gly, at 3,804,743 to 3,804,818
_RS18320
BWI76_RS18325: BWI76_RS18325 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 3,804,970 to 3,805,518
_RS18325
BWI76_RS18330: BWI76_RS18330 - excinuclease ABC subunit C, at 3,805,576 to 3,807,408
_RS18330
Position (kb)
3804
3805
3806 Strain fitness (log2 ratio)
-1
0
1 at 3803.975 kb on - strand, within BWI76_RS18305 at 3804.128 kb on + strand, within BWI76_RS18305 at 3805.581 kb on + strand at 3805.828 kb on - strand, within BWI76_RS18330 at 3805.859 kb on - strand, within BWI76_RS18330 at 3805.859 kb on - strand, within BWI76_RS18330 at 3806.088 kb on + strand, within BWI76_RS18330
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.16 mg/ml remove 3,803,975 - BWI76_RS18305 0.59 +0.0 3,804,128 + BWI76_RS18305 0.76 -0.2 3,805,581 + -0.7 3,805,828 - BWI76_RS18330 0.14 -0.1 3,805,859 - BWI76_RS18330 0.15 +0.0 3,805,859 - BWI76_RS18330 0.15 -0.5 3,806,088 + BWI76_RS18330 0.28 +0.1
Or see this region's nucleotide sequence