Experiment: Cephalothin 0.16 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18180 and BWI76_RS18185 are separated by 53 nucleotides BWI76_RS18185 and BWI76_RS18190 are separated by 39 nucleotides BWI76_RS18190 and BWI76_RS18195 overlap by 4 nucleotides
BWI76_RS18180: BWI76_RS18180 - hypothetical protein, at 3,777,514 to 3,778,332
_RS18180
BWI76_RS18185: BWI76_RS18185 - membrane protein, at 3,778,386 to 3,778,781
_RS18185
BWI76_RS18190: BWI76_RS18190 - carboxy-S-adenosyl-L-methionine synthase CmoA, at 3,778,821 to 3,779,564
_RS18190
BWI76_RS18195: BWI76_RS18195 - tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB, at 3,779,561 to 3,780,577
_RS18195
Position (kb) 3778
3779
3780 Strain fitness (log2 ratio) -2
-1
0
1 at 3777.930 kb on + strand, within BWI76_RS18180 at 3777.977 kb on - strand, within BWI76_RS18180 at 3778.454 kb on + strand, within BWI76_RS18185 at 3778.478 kb on - strand, within BWI76_RS18185 at 3778.484 kb on - strand, within BWI76_RS18185 at 3778.603 kb on - strand, within BWI76_RS18185 at 3778.605 kb on + strand, within BWI76_RS18185 at 3778.649 kb on + strand, within BWI76_RS18185 at 3778.744 kb on + strand at 3778.752 kb on - strand at 3779.110 kb on - strand, within BWI76_RS18190 at 3779.150 kb on + strand, within BWI76_RS18190 at 3779.775 kb on + strand, within BWI76_RS18195 at 3779.814 kb on + strand, within BWI76_RS18195 at 3780.107 kb on - strand, within BWI76_RS18195 at 3780.317 kb on - strand, within BWI76_RS18195
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.16 mg/ml remove 3,777,930 + BWI76_RS18180 0.51 -0.4 3,777,977 - BWI76_RS18180 0.57 +0.6 3,778,454 + BWI76_RS18185 0.17 +0.4 3,778,478 - BWI76_RS18185 0.23 -0.3 3,778,484 - BWI76_RS18185 0.25 +0.5 3,778,603 - BWI76_RS18185 0.55 -0.1 3,778,605 + BWI76_RS18185 0.55 -0.1 3,778,649 + BWI76_RS18185 0.66 +0.3 3,778,744 + -0.1 3,778,752 - +0.9 3,779,110 - BWI76_RS18190 0.39 -2.1 3,779,150 + BWI76_RS18190 0.44 -0.2 3,779,775 + BWI76_RS18195 0.21 +0.1 3,779,814 + BWI76_RS18195 0.25 -0.5 3,780,107 - BWI76_RS18195 0.54 -1.0 3,780,317 - BWI76_RS18195 0.74 -0.7
Or see this region's nucleotide sequence