Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08030

Experiment: Cephalothin 0.16 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08025 and BWI76_RS08030 are separated by 10 nucleotidesBWI76_RS08030 and BWI76_RS08035 overlap by 1 nucleotides BWI76_RS08025: BWI76_RS08025 - septal ring lytic transglycosylase RlpA, at 1,700,914 to 1,702,014 _RS08025 BWI76_RS08030: BWI76_RS08030 - cell wall shape-determining protein, at 1,702,025 to 1,703,137 _RS08030 BWI76_RS08035: BWI76_RS08035 - penicillin-binding protein 2, at 1,703,137 to 1,705,038 _RS08035 Position (kb) 1702 1703 1704Strain fitness (log2 ratio) -1 0 1 2at 1701.202 kb on + strand, within BWI76_RS08025at 1701.311 kb on - strand, within BWI76_RS08025at 1701.437 kb on + strand, within BWI76_RS08025at 1701.525 kb on + strand, within BWI76_RS08025at 1701.700 kb on - strand, within BWI76_RS08025at 1701.926 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cephalothin 0.16 mg/ml
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1,701,202 + BWI76_RS08025 0.26 +1.1
1,701,311 - BWI76_RS08025 0.36 +1.8
1,701,437 + BWI76_RS08025 0.48 +1.2
1,701,525 + BWI76_RS08025 0.55 -1.0
1,701,700 - BWI76_RS08025 0.71 +0.7
1,701,926 - +0.9

Or see this region's nucleotide sequence