Experiment: Cephalothin 0.16 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04175 and BWI76_RS04180 overlap by 60 nucleotides BWI76_RS04180 and BWI76_RS04185 are separated by 125 nucleotides BWI76_RS04185 and BWI76_RS04190 are separated by 117 nucleotides BWI76_RS04190 and BWI76_RS04195 overlap by 20 nucleotides
BWI76_RS04175: BWI76_RS04175 - energy-dependent translational throttle protein EttA, at 859,388 to 861,055
_RS04175
BWI76_RS04180: BWI76_RS04180 - hypothetical protein, at 860,996 to 861,203
_RS04180
BWI76_RS04185: BWI76_RS04185 - murein transglycosylase, at 861,329 to 863,266
_RS04185
BWI76_RS04190: BWI76_RS04190 - Trp operon repressor, at 863,384 to 863,716
_RS04190
BWI76_RS04195: BWI76_RS04195 - non-canonical purine NTP phosphatase, at 863,697 to 864,218
_RS04195
Position (kb) 861
862
863
864 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 861.021 kb on - strand at 861.062 kb on - strand, within BWI76_RS04180 at 861.082 kb on + strand, within BWI76_RS04180 at 861.118 kb on + strand, within BWI76_RS04180 at 861.126 kb on - strand, within BWI76_RS04180 at 861.131 kb on - strand, within BWI76_RS04180 at 861.133 kb on + strand, within BWI76_RS04180 at 861.176 kb on + strand, within BWI76_RS04180 at 861.293 kb on + strand at 861.530 kb on - strand, within BWI76_RS04185 at 861.687 kb on + strand, within BWI76_RS04185 at 861.768 kb on - strand, within BWI76_RS04185 at 861.778 kb on - strand, within BWI76_RS04185 at 861.835 kb on + strand, within BWI76_RS04185 at 861.897 kb on - strand, within BWI76_RS04185 at 862.016 kb on + strand, within BWI76_RS04185 at 862.085 kb on + strand, within BWI76_RS04185 at 862.128 kb on - strand, within BWI76_RS04185 at 862.161 kb on + strand, within BWI76_RS04185 at 862.182 kb on - strand, within BWI76_RS04185 at 862.458 kb on + strand, within BWI76_RS04185 at 862.495 kb on + strand, within BWI76_RS04185 at 862.695 kb on - strand, within BWI76_RS04185 at 863.062 kb on - strand, within BWI76_RS04185 at 863.231 kb on + strand at 863.241 kb on - strand at 863.283 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.16 mg/ml remove 861,021 - +1.3 861,062 - BWI76_RS04180 0.32 -0.5 861,082 + BWI76_RS04180 0.41 +1.6 861,118 + BWI76_RS04180 0.59 +1.6 861,126 - BWI76_RS04180 0.62 -0.2 861,131 - BWI76_RS04180 0.65 -0.3 861,133 + BWI76_RS04180 0.66 +0.6 861,176 + BWI76_RS04180 0.87 +0.4 861,293 + -1.4 861,530 - BWI76_RS04185 0.10 -2.7 861,687 + BWI76_RS04185 0.18 -2.2 861,768 - BWI76_RS04185 0.23 -1.9 861,778 - BWI76_RS04185 0.23 -2.7 861,835 + BWI76_RS04185 0.26 -3.9 861,897 - BWI76_RS04185 0.29 -1.6 862,016 + BWI76_RS04185 0.35 -1.8 862,085 + BWI76_RS04185 0.39 -3.6 862,128 - BWI76_RS04185 0.41 -0.7 862,161 + BWI76_RS04185 0.43 -2.3 862,182 - BWI76_RS04185 0.44 -2.7 862,458 + BWI76_RS04185 0.58 -2.1 862,495 + BWI76_RS04185 0.60 -1.3 862,695 - BWI76_RS04185 0.70 -2.4 863,062 - BWI76_RS04185 0.89 -2.2 863,231 + -2.3 863,241 - -1.7 863,283 - -1.3
Or see this region's nucleotide sequence