Experiment: Cephalothin 0.16 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02510 and BWI76_RS02515 overlap by 8 nucleotides BWI76_RS02515 and BWI76_RS02520 are separated by 16 nucleotides BWI76_RS02520 and BWI76_RS02525 are separated by 10 nucleotides
BWI76_RS02510: BWI76_RS02510 - bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase, at 513,681 to 515,207
_RS02510
BWI76_RS02515: BWI76_RS02515 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE, at 515,200 to 515,661
_RS02515
BWI76_RS02520: BWI76_RS02520 - N-acetylmuramoyl-L-alanine amidase, at 515,678 to 517,015
_RS02520
BWI76_RS02525: BWI76_RS02525 - DNA mismatch repair protein MutL, at 517,026 to 518,921
_RS02525
Position (kb) 515
516
517
518 Strain fitness (log2 ratio) -1
0
1
2 at 514.698 kb on - strand, within BWI76_RS02510 at 515.007 kb on + strand, within BWI76_RS02510 at 515.054 kb on + strand, within BWI76_RS02510 at 515.689 kb on - strand at 515.816 kb on + strand, within BWI76_RS02520 at 515.890 kb on - strand, within BWI76_RS02520 at 515.890 kb on - strand, within BWI76_RS02520 at 515.890 kb on - strand, within BWI76_RS02520 at 516.024 kb on + strand, within BWI76_RS02520 at 516.171 kb on - strand, within BWI76_RS02520 at 516.196 kb on + strand, within BWI76_RS02520 at 516.204 kb on - strand, within BWI76_RS02520 at 516.367 kb on + strand, within BWI76_RS02520 at 516.573 kb on + strand, within BWI76_RS02520 at 516.610 kb on - strand, within BWI76_RS02520 at 516.702 kb on + strand, within BWI76_RS02520 at 516.739 kb on + strand, within BWI76_RS02520 at 516.810 kb on + strand, within BWI76_RS02520 at 517.279 kb on + strand, within BWI76_RS02525 at 517.279 kb on + strand, within BWI76_RS02525 at 517.287 kb on - strand, within BWI76_RS02525 at 517.317 kb on + strand, within BWI76_RS02525 at 517.317 kb on + strand, within BWI76_RS02525 at 517.325 kb on - strand, within BWI76_RS02525 at 517.358 kb on - strand, within BWI76_RS02525 at 517.464 kb on - strand, within BWI76_RS02525 at 517.790 kb on + strand, within BWI76_RS02525 at 517.893 kb on - strand, within BWI76_RS02525 at 517.926 kb on - strand, within BWI76_RS02525
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.16 mg/ml remove 514,698 - BWI76_RS02510 0.67 -0.7 515,007 + BWI76_RS02510 0.87 -0.5 515,054 + BWI76_RS02510 0.90 -1.4 515,689 - +1.5 515,816 + BWI76_RS02520 0.10 +2.1 515,890 - BWI76_RS02520 0.16 +2.0 515,890 - BWI76_RS02520 0.16 +2.7 515,890 - BWI76_RS02520 0.16 +2.0 516,024 + BWI76_RS02520 0.26 +2.4 516,171 - BWI76_RS02520 0.37 +1.7 516,196 + BWI76_RS02520 0.39 +2.0 516,204 - BWI76_RS02520 0.39 +1.6 516,367 + BWI76_RS02520 0.51 +1.9 516,573 + BWI76_RS02520 0.67 +1.7 516,610 - BWI76_RS02520 0.70 +1.9 516,702 + BWI76_RS02520 0.77 +2.0 516,739 + BWI76_RS02520 0.79 +2.0 516,810 + BWI76_RS02520 0.85 +0.3 517,279 + BWI76_RS02525 0.13 +0.1 517,279 + BWI76_RS02525 0.13 -1.2 517,287 - BWI76_RS02525 0.14 -0.1 517,317 + BWI76_RS02525 0.15 +0.2 517,317 + BWI76_RS02525 0.15 +0.7 517,325 - BWI76_RS02525 0.16 +0.7 517,358 - BWI76_RS02525 0.18 -0.8 517,464 - BWI76_RS02525 0.23 +0.2 517,790 + BWI76_RS02525 0.40 +0.5 517,893 - BWI76_RS02525 0.46 -0.4 517,926 - BWI76_RS02525 0.47 +0.0
Or see this region's nucleotide sequence