Experiment: Cephalothin 0.16 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01990 and BWI76_RS01995 overlap by 16 nucleotides BWI76_RS01995 and BWI76_RS02000 are separated by 84 nucleotides BWI76_RS02000 and BWI76_RS02005 are separated by 258 nucleotides BWI76_RS02005 and BWI76_RS02010 are separated by 36 nucleotides
BWI76_RS01990: BWI76_RS01990 - cyclic diguanylate phosphodiesterase, at 409,135 to 410,739
_RS01990
BWI76_RS01995: BWI76_RS01995 - AraC family transcriptional regulator, at 410,724 to 411,053
_RS01995
BWI76_RS02000: BWI76_RS02000 - transcriptional regulator, at 411,138 to 411,596
_RS02000
BWI76_RS02005: BWI76_RS02005 - glutathione S-transferase, at 411,855 to 412,523
_RS02005
BWI76_RS02010: BWI76_RS02010 - hypothetical protein, at 412,560 to 412,760
_RS02010
Position (kb) 411
412 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 410.141 kb on - strand, within BWI76_RS01990 at 410.142 kb on - strand, within BWI76_RS01990 at 410.279 kb on - strand, within BWI76_RS01990 at 410.291 kb on + strand, within BWI76_RS01990 at 410.413 kb on + strand, within BWI76_RS01990 at 410.422 kb on + strand, within BWI76_RS01990 at 410.430 kb on - strand, within BWI76_RS01990 at 410.625 kb on - strand at 410.915 kb on - strand, within BWI76_RS01995 at 411.091 kb on - strand at 411.314 kb on + strand, within BWI76_RS02000 at 411.385 kb on + strand, within BWI76_RS02000 at 411.393 kb on - strand, within BWI76_RS02000 at 411.393 kb on - strand, within BWI76_RS02000 at 411.454 kb on + strand, within BWI76_RS02000 at 411.454 kb on + strand, within BWI76_RS02000 at 411.454 kb on + strand, within BWI76_RS02000 at 411.454 kb on + strand, within BWI76_RS02000 at 411.454 kb on + strand, within BWI76_RS02000 at 411.462 kb on - strand, within BWI76_RS02000 at 411.462 kb on - strand, within BWI76_RS02000 at 411.462 kb on - strand, within BWI76_RS02000 at 411.462 kb on - strand, within BWI76_RS02000 at 411.699 kb on + strand at 411.699 kb on + strand at 411.699 kb on + strand at 411.699 kb on + strand at 411.702 kb on - strand at 411.707 kb on - strand at 411.707 kb on - strand at 411.707 kb on - strand at 411.707 kb on - strand at 411.727 kb on + strand at 411.864 kb on - strand at 411.943 kb on - strand, within BWI76_RS02005 at 411.951 kb on - strand, within BWI76_RS02005 at 411.953 kb on + strand, within BWI76_RS02005 at 411.959 kb on + strand, within BWI76_RS02005 at 412.056 kb on + strand, within BWI76_RS02005 at 412.078 kb on + strand, within BWI76_RS02005 at 412.085 kb on - strand, within BWI76_RS02005 at 412.145 kb on + strand, within BWI76_RS02005 at 412.150 kb on + strand, within BWI76_RS02005 at 412.213 kb on - strand, within BWI76_RS02005 at 412.253 kb on + strand, within BWI76_RS02005 at 412.261 kb on - strand, within BWI76_RS02005 at 412.285 kb on - strand, within BWI76_RS02005 at 412.322 kb on + strand, within BWI76_RS02005 at 412.461 kb on - strand at 412.463 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.16 mg/ml remove 410,141 - BWI76_RS01990 0.63 +1.2 410,142 - BWI76_RS01990 0.63 +1.4 410,279 - BWI76_RS01990 0.71 +1.1 410,291 + BWI76_RS01990 0.72 +1.6 410,413 + BWI76_RS01990 0.80 +1.4 410,422 + BWI76_RS01990 0.80 +1.2 410,430 - BWI76_RS01990 0.81 +1.2 410,625 - +1.3 410,915 - BWI76_RS01995 0.58 -0.2 411,091 - -0.1 411,314 + BWI76_RS02000 0.38 -0.1 411,385 + BWI76_RS02000 0.54 +0.2 411,393 - BWI76_RS02000 0.56 +0.3 411,393 - BWI76_RS02000 0.56 -0.6 411,454 + BWI76_RS02000 0.69 +0.4 411,454 + BWI76_RS02000 0.69 +0.6 411,454 + BWI76_RS02000 0.69 +0.4 411,454 + BWI76_RS02000 0.69 -0.1 411,454 + BWI76_RS02000 0.69 -1.3 411,462 - BWI76_RS02000 0.71 -0.1 411,462 - BWI76_RS02000 0.71 +2.8 411,462 - BWI76_RS02000 0.71 -0.6 411,462 - BWI76_RS02000 0.71 -0.7 411,699 + +0.2 411,699 + +0.3 411,699 + -0.5 411,699 + +0.3 411,702 - -1.2 411,707 - +0.1 411,707 - +0.2 411,707 - -1.1 411,707 - +0.7 411,727 + +1.3 411,864 - +3.7 411,943 - BWI76_RS02005 0.13 +0.7 411,951 - BWI76_RS02005 0.14 +0.9 411,953 + BWI76_RS02005 0.15 +1.8 411,959 + BWI76_RS02005 0.16 -0.3 412,056 + BWI76_RS02005 0.30 +1.5 412,078 + BWI76_RS02005 0.33 +1.4 412,085 - BWI76_RS02005 0.34 +0.6 412,145 + BWI76_RS02005 0.43 +1.4 412,150 + BWI76_RS02005 0.44 -0.2 412,213 - BWI76_RS02005 0.54 +0.7 412,253 + BWI76_RS02005 0.59 +0.3 412,261 - BWI76_RS02005 0.61 -2.0 412,285 - BWI76_RS02005 0.64 +0.0 412,322 + BWI76_RS02005 0.70 +1.5 412,461 - +0.2 412,463 + +1.1
Or see this region's nucleotide sequence