Experiment: MoLS4 with Chlorite 6.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiE and DvMF_3008 are separated by 227 nucleotides DvMF_3008 and DvMF_3009 are separated by 99 nucleotides DvMF_3009 and DvMF_3010 are separated by 368 nucleotides
DvMF_3007: thiE - thiamine-phosphate pyrophosphorylase (RefSeq), at 3,794,425 to 3,795,066
thiE
DvMF_3008: DvMF_3008 - Dinitrogenase iron-molybdenum cofactor biosynthesis protein (RefSeq), at 3,795,294 to 3,795,629
_3008
DvMF_3009: DvMF_3009 - oxidoreductase FAD/NAD(P)-binding domain protein (RefSeq), at 3,795,729 to 3,796,493
_3009
DvMF_3010: DvMF_3010 - sigma54 specific transcriptional regulator, Fis family (RefSeq), at 3,796,862 to 3,798,667
_3010
Position (kb)
3795
3796
3797 Strain fitness (log2 ratio)
-1
0
1 at 3794.942 kb on - strand, within thiE at 3794.985 kb on - strand, within thiE at 3795.080 kb on - strand at 3795.129 kb on - strand at 3795.200 kb on + strand at 3795.221 kb on + strand at 3795.221 kb on + strand at 3795.701 kb on + strand at 3795.712 kb on + strand at 3795.720 kb on - strand at 3795.834 kb on + strand, within DvMF_3009 at 3795.925 kb on + strand, within DvMF_3009 at 3796.560 kb on - strand at 3796.630 kb on + strand at 3797.297 kb on - strand, within DvMF_3010
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with Chlorite 6.25 mM remove 3,794,942 - thiE DvMF_3007 0.81 -0.7 3,794,985 - thiE DvMF_3007 0.87 -1.1 3,795,080 - -0.5 3,795,129 - -0.4 3,795,200 + -0.8 3,795,221 + -0.2 3,795,221 + -0.3 3,795,701 + -0.9 3,795,712 + +0.7 3,795,720 - -0.5 3,795,834 + DvMF_3009 0.14 -0.6 3,795,925 + DvMF_3009 0.26 +0.1 3,796,560 - -1.4 3,796,630 + +0.4 3,797,297 - DvMF_3010 0.24 -0.3
Or see this region's nucleotide sequence