Experiment: marine broth
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_539 and HP15_540 overlap by 1 nucleotides HP15_540 and HP15_541 are separated by 43 nucleotides HP15_541 and HP15_542 are separated by 21 nucleotides
HP15_539: HP15_539 - 2-isopropylmalate synthase, at 561,614 to 562,372
_539
HP15_540: HP15_540 - ribosomal-protein-alanine acetyltransferase, at 562,372 to 562,857
_540
HP15_541: HP15_541 - peptide chain release factor 3, at 562,901 to 564,526
_541
HP15_542: HP15_542 - TatD-related deoxyribonuclease, at 564,548 to 565,333
_542
Position (kb) 562
563
564
565 Strain fitness (log2 ratio) -2
-1
0
1
2 at 562.445 kb on + strand, within HP15_540 at 562.533 kb on + strand, within HP15_540 at 562.533 kb on + strand, within HP15_540 at 562.533 kb on + strand, within HP15_540 at 562.767 kb on - strand, within HP15_540 at 562.787 kb on + strand, within HP15_540 at 562.856 kb on + strand at 562.955 kb on - strand at 563.017 kb on + strand at 563.167 kb on - strand, within HP15_541 at 563.260 kb on - strand, within HP15_541 at 563.292 kb on - strand, within HP15_541 at 563.300 kb on - strand, within HP15_541 at 563.525 kb on + strand, within HP15_541 at 563.635 kb on + strand, within HP15_541 at 563.643 kb on - strand, within HP15_541 at 563.643 kb on - strand, within HP15_541 at 563.643 kb on - strand, within HP15_541 at 563.885 kb on - strand, within HP15_541 at 564.070 kb on - strand, within HP15_541 at 564.311 kb on + strand, within HP15_541 at 564.362 kb on - strand, within HP15_541 at 564.457 kb on - strand at 564.646 kb on - strand, within HP15_542 at 564.674 kb on + strand, within HP15_542 at 564.674 kb on + strand, within HP15_542 at 564.718 kb on + strand, within HP15_542 at 564.739 kb on + strand, within HP15_542 at 564.936 kb on - strand, within HP15_542 at 565.152 kb on - strand, within HP15_542 at 565.251 kb on - strand, within HP15_542
Per-strain Table
Position Strand Gene LocusTag Fraction marine broth remove 562,445 + HP15_540 0.15 -0.0 562,533 + HP15_540 0.33 +0.1 562,533 + HP15_540 0.33 +0.5 562,533 + HP15_540 0.33 -0.5 562,767 - HP15_540 0.81 -0.2 562,787 + HP15_540 0.85 +0.8 562,856 + +0.1 562,955 - +0.3 563,017 + -0.6 563,167 - HP15_541 0.16 -1.2 563,260 - HP15_541 0.22 -0.2 563,292 - HP15_541 0.24 +0.5 563,300 - HP15_541 0.25 -0.2 563,525 + HP15_541 0.38 -0.0 563,635 + HP15_541 0.45 -1.0 563,643 - HP15_541 0.46 -1.8 563,643 - HP15_541 0.46 -0.8 563,643 - HP15_541 0.46 -0.6 563,885 - HP15_541 0.61 -0.2 564,070 - HP15_541 0.72 -0.6 564,311 + HP15_541 0.87 +0.5 564,362 - HP15_541 0.90 +2.1 564,457 - +1.1 564,646 - HP15_542 0.12 +0.2 564,674 + HP15_542 0.16 +0.1 564,674 + HP15_542 0.16 +0.2 564,718 + HP15_542 0.22 +1.7 564,739 + HP15_542 0.24 +1.1 564,936 - HP15_542 0.49 -0.3 565,152 - HP15_542 0.77 +0.0 565,251 - HP15_542 0.89 +1.1
Or see this region's nucleotide sequence