Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13460 and BWI76_RS13465 are separated by 16 nucleotides BWI76_RS13465 and BWI76_RS13470 are separated by 8 nucleotides BWI76_RS13470 and BWI76_RS13475 overlap by 4 nucleotides BWI76_RS13475 and BWI76_RS13480 are separated by 276 nucleotides
BWI76_RS13460: BWI76_RS13460 - ABC transporter substrate-binding protein, at 2,807,184 to 2,808,782
_RS13460
BWI76_RS13465: BWI76_RS13465 - hypothetical protein, at 2,808,799 to 2,809,032
_RS13465
BWI76_RS13470: BWI76_RS13470 - transporter, at 2,809,041 to 2,809,490
_RS13470
BWI76_RS13475: BWI76_RS13475 - N-acetyltransferase, at 2,809,487 to 2,810,005
_RS13475
BWI76_RS13480: BWI76_RS13480 - XRE family transcriptional regulator, at 2,810,282 to 2,810,857
_RS13480
Position (kb)
2809
2810 Strain fitness (log2 ratio)
-1
0
1
2 at 2808.065 kb on + strand, within BWI76_RS13460 at 2808.324 kb on + strand, within BWI76_RS13460 at 2808.676 kb on + strand at 2808.909 kb on - strand, within BWI76_RS13465 at 2809.194 kb on + strand, within BWI76_RS13470 at 2809.585 kb on + strand at 2809.723 kb on - strand, within BWI76_RS13475 at 2810.105 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,808,065 + BWI76_RS13460 0.55 -0.6 2,808,324 + BWI76_RS13460 0.71 +2.5 2,808,676 + -0.2 2,808,909 - BWI76_RS13465 0.47 +0.5 2,809,194 + BWI76_RS13470 0.34 -0.8 2,809,585 + +1.2 2,809,723 - BWI76_RS13475 0.45 +0.8 2,810,105 + -0.4
Or see this region's nucleotide sequence