Experiment: H2 N-free with 10mM Gly-D-Asp
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02715 and MMP_RS02720 are separated by 238 nucleotides MMP_RS02720 and MMP_RS02725 are separated by 10 nucleotides MMP_RS02725 and MMP_RS02730 are separated by 123 nucleotides
MMP_RS02715: MMP_RS02715 - molybdopterin molybdotransferase MoeA, at 518,774 to 519,895
_RS02715
MMP_RS02720: MMP_RS02720 - molybdate ABC transporter substrate-binding protein, at 520,134 to 520,910
_RS02720
MMP_RS02725: MMP_RS02725 - molybdate ABC transporter permease subunit, at 520,921 to 521,586
_RS02725
MMP_RS02730: MMP_RS02730 - ModD protein, at 521,710 to 522,561
_RS02730
Position (kb) 520
521 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 519.386 kb on - strand, within MMP_RS02715 at 519.410 kb on - strand, within MMP_RS02715 at 519.468 kb on + strand, within MMP_RS02715 at 519.468 kb on + strand, within MMP_RS02715 at 519.532 kb on - strand, within MMP_RS02715 at 519.593 kb on - strand, within MMP_RS02715 at 519.593 kb on - strand, within MMP_RS02715 at 519.831 kb on + strand at 519.918 kb on - strand at 519.976 kb on + strand at 520.013 kb on - strand at 520.013 kb on - strand at 520.014 kb on + strand at 520.045 kb on - strand at 520.045 kb on - strand at 520.046 kb on + strand at 520.046 kb on + strand at 520.050 kb on + strand at 520.526 kb on + strand, within MMP_RS02720 at 520.678 kb on + strand, within MMP_RS02720 at 520.711 kb on - strand, within MMP_RS02720 at 520.852 kb on + strand at 520.950 kb on - strand at 520.971 kb on + strand at 521.004 kb on - strand, within MMP_RS02725 at 521.004 kb on - strand, within MMP_RS02725 at 521.063 kb on - strand, within MMP_RS02725 at 521.064 kb on + strand, within MMP_RS02725 at 521.096 kb on - strand, within MMP_RS02725 at 521.105 kb on - strand, within MMP_RS02725 at 521.291 kb on + strand, within MMP_RS02725 at 521.369 kb on - strand, within MMP_RS02725 at 521.377 kb on + strand, within MMP_RS02725 at 521.463 kb on - strand, within MMP_RS02725 at 521.511 kb on + strand, within MMP_RS02725 at 521.524 kb on + strand at 521.546 kb on - strand at 521.546 kb on - strand at 521.547 kb on + strand at 521.548 kb on - strand at 521.548 kb on - strand at 521.548 kb on - strand at 521.548 kb on - strand at 521.548 kb on - strand at 521.549 kb on + strand at 521.549 kb on + strand at 521.549 kb on + strand at 521.553 kb on + strand at 521.572 kb on + strand at 521.621 kb on + strand at 521.621 kb on + strand at 521.621 kb on + strand at 521.623 kb on + strand at 521.635 kb on - strand at 521.635 kb on - strand at 521.636 kb on + strand at 521.636 kb on + strand at 521.636 kb on + strand at 521.672 kb on - strand at 521.675 kb on - strand at 521.676 kb on + strand at 521.680 kb on + strand at 521.709 kb on - strand at 521.710 kb on + strand at 521.710 kb on + strand at 521.719 kb on - strand at 521.719 kb on - strand at 521.825 kb on + strand, within MMP_RS02730 at 521.876 kb on - strand, within MMP_RS02730 at 521.876 kb on - strand, within MMP_RS02730 at 521.877 kb on + strand, within MMP_RS02730
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Gly-D-Asp remove 519,386 - MMP_RS02715 0.55 -0.9 519,410 - MMP_RS02715 0.57 -0.0 519,468 + MMP_RS02715 0.62 -0.1 519,468 + MMP_RS02715 0.62 +0.2 519,532 - MMP_RS02715 0.68 -0.3 519,593 - MMP_RS02715 0.73 -0.6 519,593 - MMP_RS02715 0.73 +0.1 519,831 + -0.4 519,918 - +1.1 519,976 + -0.4 520,013 - +0.1 520,013 - -0.1 520,014 + +0.7 520,045 - +0.9 520,045 - +0.3 520,046 + +0.6 520,046 + -1.5 520,050 + +0.3 520,526 + MMP_RS02720 0.50 +0.8 520,678 + MMP_RS02720 0.70 -0.0 520,711 - MMP_RS02720 0.74 -0.7 520,852 + -0.4 520,950 - +0.6 520,971 + +1.4 521,004 - MMP_RS02725 0.12 +0.3 521,004 - MMP_RS02725 0.12 +0.8 521,063 - MMP_RS02725 0.21 -0.9 521,064 + MMP_RS02725 0.21 -0.5 521,096 - MMP_RS02725 0.26 -1.1 521,105 - MMP_RS02725 0.28 +0.3 521,291 + MMP_RS02725 0.56 -1.4 521,369 - MMP_RS02725 0.67 +0.0 521,377 + MMP_RS02725 0.68 -0.1 521,463 - MMP_RS02725 0.81 +0.8 521,511 + MMP_RS02725 0.89 -0.5 521,524 + +0.2 521,546 - +0.0 521,546 - +0.3 521,547 + +0.5 521,548 - -1.0 521,548 - +0.5 521,548 - -0.6 521,548 - -1.6 521,548 - -0.5 521,549 + -0.0 521,549 + +0.4 521,549 + +0.1 521,553 + +0.7 521,572 + +0.4 521,621 + -1.5 521,621 + -1.3 521,621 + -0.5 521,623 + -0.6 521,635 - -3.0 521,635 - -3.5 521,636 + -1.6 521,636 + -1.8 521,636 + -1.4 521,672 - -2.9 521,675 - -3.7 521,676 + -1.9 521,680 + -0.7 521,709 - -2.7 521,710 + -4.0 521,710 + -2.8 521,719 - -2.7 521,719 - -4.2 521,825 + MMP_RS02730 0.13 -2.8 521,876 - MMP_RS02730 0.19 -2.2 521,876 - MMP_RS02730 0.19 -3.0 521,877 + MMP_RS02730 0.20 -2.5
Or see this region's nucleotide sequence