Experiment: H2 N-free with 10mM Gly-D-Asp
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00050 and MMP_RS00055 are separated by 81 nucleotides MMP_RS00055 and MMP_RS00060 are separated by 127 nucleotides
MMP_RS00050: MMP_RS00050 - AarF/UbiB family protein, at 12,647 to 13,543
_RS00050
MMP_RS00055: MMP_RS00055 - DUF2254 domain-containing protein, at 13,625 to 15,208
_RS00055
MMP_RS00060: MMP_RS00060 - 3-dehydroquinate synthase II, at 15,336 to 16,421
_RS00060
Position (kb) 13
14
15
16 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 12.653 kb on + strand at 12.665 kb on - strand at 12.668 kb on + strand at 12.681 kb on - strand at 12.682 kb on + strand at 12.685 kb on - strand at 12.686 kb on + strand at 12.689 kb on - strand at 12.689 kb on - strand at 12.690 kb on + strand at 12.690 kb on + strand at 12.695 kb on + strand at 12.695 kb on + strand at 12.695 kb on + strand at 12.695 kb on + strand at 12.695 kb on + strand at 12.695 kb on + strand at 12.699 kb on + strand at 12.729 kb on - strand at 12.758 kb on - strand, within MMP_RS00050 at 12.777 kb on - strand, within MMP_RS00050 at 12.782 kb on - strand, within MMP_RS00050 at 12.782 kb on - strand, within MMP_RS00050 at 12.882 kb on + strand, within MMP_RS00050 at 12.924 kb on - strand, within MMP_RS00050 at 13.004 kb on - strand, within MMP_RS00050 at 13.073 kb on - strand, within MMP_RS00050 at 13.145 kb on + strand, within MMP_RS00050 at 13.168 kb on + strand, within MMP_RS00050 at 13.247 kb on + strand, within MMP_RS00050 at 13.324 kb on - strand, within MMP_RS00050 at 13.431 kb on + strand, within MMP_RS00050 at 13.595 kb on + strand at 13.598 kb on + strand at 13.598 kb on + strand at 13.608 kb on - strand at 13.608 kb on - strand at 13.630 kb on - strand at 13.631 kb on + strand at 13.703 kb on + strand at 13.787 kb on - strand, within MMP_RS00055 at 13.835 kb on - strand, within MMP_RS00055 at 13.849 kb on + strand, within MMP_RS00055 at 13.991 kb on - strand, within MMP_RS00055 at 13.994 kb on + strand, within MMP_RS00055 at 14.033 kb on - strand, within MMP_RS00055 at 14.264 kb on - strand, within MMP_RS00055 at 14.272 kb on + strand, within MMP_RS00055 at 14.283 kb on - strand, within MMP_RS00055 at 14.327 kb on - strand, within MMP_RS00055 at 14.328 kb on + strand, within MMP_RS00055 at 14.328 kb on + strand, within MMP_RS00055 at 14.334 kb on - strand, within MMP_RS00055 at 14.345 kb on - strand, within MMP_RS00055 at 14.420 kb on - strand, within MMP_RS00055 at 14.444 kb on - strand, within MMP_RS00055 at 14.448 kb on - strand, within MMP_RS00055 at 14.575 kb on - strand, within MMP_RS00055 at 14.576 kb on + strand, within MMP_RS00055 at 14.576 kb on + strand, within MMP_RS00055 at 14.576 kb on + strand, within MMP_RS00055 at 14.626 kb on + strand, within MMP_RS00055 at 14.742 kb on - strand, within MMP_RS00055 at 14.801 kb on + strand, within MMP_RS00055 at 14.801 kb on + strand, within MMP_RS00055 at 14.967 kb on + strand, within MMP_RS00055 at 15.046 kb on - strand, within MMP_RS00055 at 15.047 kb on + strand, within MMP_RS00055 at 15.131 kb on - strand at 15.172 kb on - strand at 15.173 kb on + strand at 15.177 kb on - strand at 15.177 kb on - strand at 15.234 kb on + strand at 15.237 kb on - strand at 15.237 kb on - strand at 15.238 kb on + strand at 15.242 kb on - strand at 15.242 kb on - strand at 15.242 kb on - strand at 15.246 kb on - strand at 15.247 kb on + strand at 15.247 kb on + strand at 15.247 kb on + strand at 15.253 kb on + strand at 15.267 kb on + strand at 15.279 kb on - strand at 15.280 kb on + strand at 15.515 kb on + strand, within MMP_RS00060 at 15.590 kb on + strand, within MMP_RS00060 at 16.082 kb on + strand, within MMP_RS00060
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Gly-D-Asp remove 12,653 + -1.8 12,665 - -2.6 12,668 + -3.2 12,681 - -0.4 12,682 + -3.1 12,685 - -1.8 12,686 + -1.6 12,689 - -2.2 12,689 - -2.2 12,690 + -3.0 12,690 + -3.4 12,695 + -0.8 12,695 + -2.0 12,695 + -1.4 12,695 + -1.8 12,695 + -1.7 12,695 + +0.3 12,699 + +0.8 12,729 - -1.6 12,758 - MMP_RS00050 0.12 -0.6 12,777 - MMP_RS00050 0.14 -0.7 12,782 - MMP_RS00050 0.15 +0.2 12,782 - MMP_RS00050 0.15 +1.7 12,882 + MMP_RS00050 0.26 +1.1 12,924 - MMP_RS00050 0.31 +0.0 13,004 - MMP_RS00050 0.40 -1.2 13,073 - MMP_RS00050 0.47 +0.5 13,145 + MMP_RS00050 0.56 -0.6 13,168 + MMP_RS00050 0.58 -1.1 13,247 + MMP_RS00050 0.67 -0.6 13,324 - MMP_RS00050 0.75 +1.7 13,431 + MMP_RS00050 0.87 +0.6 13,595 + +0.8 13,598 + +0.5 13,598 + +1.5 13,608 - +0.8 13,608 - +0.2 13,630 - +0.4 13,631 + +0.4 13,703 + +0.6 13,787 - MMP_RS00055 0.10 +0.6 13,835 - MMP_RS00055 0.13 +1.5 13,849 + MMP_RS00055 0.14 +1.4 13,991 - MMP_RS00055 0.23 +0.7 13,994 + MMP_RS00055 0.23 +0.6 14,033 - MMP_RS00055 0.26 +1.6 14,264 - MMP_RS00055 0.40 +1.3 14,272 + MMP_RS00055 0.41 +1.1 14,283 - MMP_RS00055 0.42 +0.7 14,327 - MMP_RS00055 0.44 +0.7 14,328 + MMP_RS00055 0.44 +1.7 14,328 + MMP_RS00055 0.44 +0.5 14,334 - MMP_RS00055 0.45 +1.5 14,345 - MMP_RS00055 0.45 +1.3 14,420 - MMP_RS00055 0.50 +1.1 14,444 - MMP_RS00055 0.52 +2.0 14,448 - MMP_RS00055 0.52 +1.1 14,575 - MMP_RS00055 0.60 +2.0 14,576 + MMP_RS00055 0.60 +0.8 14,576 + MMP_RS00055 0.60 +2.0 14,576 + MMP_RS00055 0.60 +1.2 14,626 + MMP_RS00055 0.63 +1.2 14,742 - MMP_RS00055 0.71 -0.2 14,801 + MMP_RS00055 0.74 +0.9 14,801 + MMP_RS00055 0.74 +1.7 14,967 + MMP_RS00055 0.85 +0.4 15,046 - MMP_RS00055 0.90 +1.9 15,047 + MMP_RS00055 0.90 +1.1 15,131 - +0.7 15,172 - +1.5 15,173 + +1.0 15,177 - +0.8 15,177 - +0.7 15,234 + +0.0 15,237 - +0.1 15,237 - +1.0 15,238 + +1.2 15,242 - +0.7 15,242 - -0.2 15,242 - -0.3 15,246 - +1.3 15,247 + +0.8 15,247 + +0.8 15,247 + +1.8 15,253 + +0.4 15,267 + -2.5 15,279 - -1.9 15,280 + -0.1 15,515 + MMP_RS00060 0.16 -0.7 15,590 + MMP_RS00060 0.23 -0.8 16,082 + MMP_RS00060 0.69 -2.4
Or see this region's nucleotide sequence