Experiment: Glycine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c30440 and PGA1_c30450 are separated by 30 nucleotides PGA1_c30450 and PGA1_c30460 are separated by 88 nucleotides PGA1_c30460 and PGA1_c30470 are separated by 10 nucleotides
PGA1_c30440: PGA1_c30440 - putative NADP-dependent oxidoreductase, at 3,179,645 to 3,180,676
_c30440
PGA1_c30450: PGA1_c30450 - putative DJ-1/PfpI family protein, at 3,180,707 to 3,181,381
_c30450
PGA1_c30460: PGA1_c30460 - D-alanine aminotransferase Dat, at 3,181,470 to 3,182,330
_c30460
PGA1_c30470: PGA1_c30470 - mandelate racemase / muconate lactonizing enzyme, at 3,182,341 to 3,183,306
_c30470
Position (kb)
3181
3182
3183 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3180.476 kb on + strand, within PGA1_c30440 at 3180.486 kb on + strand, within PGA1_c30440 at 3180.549 kb on + strand, within PGA1_c30440 at 3180.554 kb on - strand, within PGA1_c30440 at 3180.557 kb on - strand, within PGA1_c30440 at 3180.574 kb on - strand at 3180.594 kb on + strand at 3180.601 kb on - strand at 3180.601 kb on - strand at 3180.636 kb on - strand at 3180.646 kb on + strand at 3180.654 kb on + strand at 3180.683 kb on - strand at 3180.730 kb on - strand at 3180.841 kb on + strand, within PGA1_c30450 at 3180.843 kb on - strand, within PGA1_c30450 at 3180.895 kb on + strand, within PGA1_c30450 at 3180.919 kb on + strand, within PGA1_c30450 at 3180.949 kb on - strand, within PGA1_c30450 at 3180.954 kb on - strand, within PGA1_c30450 at 3180.997 kb on + strand, within PGA1_c30450 at 3180.997 kb on + strand, within PGA1_c30450 at 3180.997 kb on + strand, within PGA1_c30450 at 3180.997 kb on + strand, within PGA1_c30450 at 3180.997 kb on + strand, within PGA1_c30450 at 3181.005 kb on - strand, within PGA1_c30450 at 3181.005 kb on - strand, within PGA1_c30450 at 3181.005 kb on - strand, within PGA1_c30450 at 3181.005 kb on - strand, within PGA1_c30450 at 3181.005 kb on - strand, within PGA1_c30450 at 3181.005 kb on - strand, within PGA1_c30450 at 3181.030 kb on - strand, within PGA1_c30450 at 3181.163 kb on + strand, within PGA1_c30450 at 3181.208 kb on + strand, within PGA1_c30450 at 3181.211 kb on - strand, within PGA1_c30450 at 3181.213 kb on + strand, within PGA1_c30450 at 3181.221 kb on - strand, within PGA1_c30450 at 3181.223 kb on - strand, within PGA1_c30450 at 3181.293 kb on + strand, within PGA1_c30450 at 3181.293 kb on + strand, within PGA1_c30450 at 3181.301 kb on - strand, within PGA1_c30450 at 3181.333 kb on - strand at 3181.333 kb on - strand at 3181.349 kb on - strand at 3181.375 kb on + strand at 3181.394 kb on + strand at 3181.422 kb on + strand at 3181.427 kb on + strand at 3181.540 kb on + strand at 3181.558 kb on - strand, within PGA1_c30460 at 3181.589 kb on - strand, within PGA1_c30460 at 3181.595 kb on - strand, within PGA1_c30460 at 3181.683 kb on + strand, within PGA1_c30460 at 3181.683 kb on + strand, within PGA1_c30460 at 3181.683 kb on + strand, within PGA1_c30460 at 3181.691 kb on - strand, within PGA1_c30460 at 3181.691 kb on - strand, within PGA1_c30460 at 3181.705 kb on + strand, within PGA1_c30460 at 3181.708 kb on - strand, within PGA1_c30460 at 3181.708 kb on - strand, within PGA1_c30460 at 3181.770 kb on - strand, within PGA1_c30460 at 3181.782 kb on + strand, within PGA1_c30460 at 3181.833 kb on - strand, within PGA1_c30460 at 3181.856 kb on + strand, within PGA1_c30460 at 3181.866 kb on + strand, within PGA1_c30460 at 3181.869 kb on - strand, within PGA1_c30460 at 3181.871 kb on + strand, within PGA1_c30460 at 3181.879 kb on + strand, within PGA1_c30460 at 3181.879 kb on + strand, within PGA1_c30460 at 3181.879 kb on + strand, within PGA1_c30460 at 3181.879 kb on + strand, within PGA1_c30460 at 3181.879 kb on + strand, within PGA1_c30460 at 3181.879 kb on + strand, within PGA1_c30460 at 3181.879 kb on + strand, within PGA1_c30460 at 3181.887 kb on - strand, within PGA1_c30460 at 3181.887 kb on - strand, within PGA1_c30460 at 3181.887 kb on - strand, within PGA1_c30460 at 3181.935 kb on + strand, within PGA1_c30460 at 3181.966 kb on + strand, within PGA1_c30460 at 3181.966 kb on + strand, within PGA1_c30460 at 3181.966 kb on + strand, within PGA1_c30460 at 3181.966 kb on + strand, within PGA1_c30460 at 3181.974 kb on - strand, within PGA1_c30460 at 3181.974 kb on - strand, within PGA1_c30460 at 3181.974 kb on - strand, within PGA1_c30460 at 3182.008 kb on + strand, within PGA1_c30460 at 3182.047 kb on + strand, within PGA1_c30460 at 3182.047 kb on + strand, within PGA1_c30460 at 3182.050 kb on - strand, within PGA1_c30460 at 3182.055 kb on - strand, within PGA1_c30460 at 3182.055 kb on - strand, within PGA1_c30460 at 3182.055 kb on - strand, within PGA1_c30460 at 3182.064 kb on + strand, within PGA1_c30460 at 3182.121 kb on - strand, within PGA1_c30460 at 3182.131 kb on + strand, within PGA1_c30460 at 3182.136 kb on + strand, within PGA1_c30460 at 3182.219 kb on - strand, within PGA1_c30460 at 3182.247 kb on - strand at 3182.318 kb on + strand at 3182.339 kb on + strand at 3182.347 kb on + strand at 3182.347 kb on - strand at 3182.407 kb on + strand at 3182.407 kb on + strand at 3182.446 kb on - strand, within PGA1_c30470 at 3182.487 kb on + strand, within PGA1_c30470 at 3182.517 kb on + strand, within PGA1_c30470 at 3182.576 kb on + strand, within PGA1_c30470 at 3182.662 kb on + strand, within PGA1_c30470 at 3182.662 kb on + strand, within PGA1_c30470 at 3182.670 kb on - strand, within PGA1_c30470 at 3182.773 kb on + strand, within PGA1_c30470 at 3182.928 kb on + strand, within PGA1_c30470 at 3183.085 kb on + strand, within PGA1_c30470 at 3183.127 kb on - strand, within PGA1_c30470 at 3183.150 kb on - strand, within PGA1_c30470 at 3183.293 kb on + strand at 3183.306 kb on - strand at 3183.311 kb on - strand at 3183.313 kb on + strand at 3183.323 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glycine (C) remove 3,180,476 + PGA1_c30440 0.81 -1.7 3,180,486 + PGA1_c30440 0.81 -0.4 3,180,549 + PGA1_c30440 0.88 +0.3 3,180,554 - PGA1_c30440 0.88 -0.2 3,180,557 - PGA1_c30440 0.88 +0.4 3,180,574 - +0.9 3,180,594 + -0.0 3,180,601 - -0.5 3,180,601 - +0.3 3,180,636 - -0.7 3,180,646 + -1.1 3,180,654 + +0.2 3,180,683 - +0.2 3,180,730 - -0.1 3,180,841 + PGA1_c30450 0.20 -0.7 3,180,843 - PGA1_c30450 0.20 +0.4 3,180,895 + PGA1_c30450 0.28 +0.6 3,180,919 + PGA1_c30450 0.31 -1.0 3,180,949 - PGA1_c30450 0.36 +0.1 3,180,954 - PGA1_c30450 0.37 +0.7 3,180,997 + PGA1_c30450 0.43 +0.0 3,180,997 + PGA1_c30450 0.43 +0.7 3,180,997 + PGA1_c30450 0.43 +1.0 3,180,997 + PGA1_c30450 0.43 -0.6 3,180,997 + PGA1_c30450 0.43 -0.7 3,181,005 - PGA1_c30450 0.44 +0.1 3,181,005 - PGA1_c30450 0.44 -0.4 3,181,005 - PGA1_c30450 0.44 +0.1 3,181,005 - PGA1_c30450 0.44 -0.2 3,181,005 - PGA1_c30450 0.44 -0.2 3,181,005 - PGA1_c30450 0.44 -0.4 3,181,030 - PGA1_c30450 0.48 +0.3 3,181,163 + PGA1_c30450 0.68 +0.0 3,181,208 + PGA1_c30450 0.74 -1.6 3,181,211 - PGA1_c30450 0.75 -1.5 3,181,213 + PGA1_c30450 0.75 +0.6 3,181,221 - PGA1_c30450 0.76 -0.6 3,181,223 - PGA1_c30450 0.76 -0.1 3,181,293 + PGA1_c30450 0.87 -0.6 3,181,293 + PGA1_c30450 0.87 -1.6 3,181,301 - PGA1_c30450 0.88 +0.0 3,181,333 - -1.7 3,181,333 - -0.5 3,181,349 - +0.5 3,181,375 + +0.1 3,181,394 + -0.3 3,181,422 + +0.3 3,181,427 + -0.0 3,181,540 + -0.4 3,181,558 - PGA1_c30460 0.10 -0.2 3,181,589 - PGA1_c30460 0.14 -0.3 3,181,595 - PGA1_c30460 0.15 -0.9 3,181,683 + PGA1_c30460 0.25 -0.4 3,181,683 + PGA1_c30460 0.25 -1.4 3,181,683 + PGA1_c30460 0.25 -1.2 3,181,691 - PGA1_c30460 0.26 -0.6 3,181,691 - PGA1_c30460 0.26 -1.7 3,181,705 + PGA1_c30460 0.27 -1.2 3,181,708 - PGA1_c30460 0.28 -0.3 3,181,708 - PGA1_c30460 0.28 -1.0 3,181,770 - PGA1_c30460 0.35 -1.3 3,181,782 + PGA1_c30460 0.36 -0.9 3,181,833 - PGA1_c30460 0.42 -0.1 3,181,856 + PGA1_c30460 0.45 +0.2 3,181,866 + PGA1_c30460 0.46 -1.7 3,181,869 - PGA1_c30460 0.46 -1.6 3,181,871 + PGA1_c30460 0.47 -2.0 3,181,879 + PGA1_c30460 0.48 +0.9 3,181,879 + PGA1_c30460 0.48 -1.0 3,181,879 + PGA1_c30460 0.48 -0.2 3,181,879 + PGA1_c30460 0.48 +0.0 3,181,879 + PGA1_c30460 0.48 -0.2 3,181,879 + PGA1_c30460 0.48 -0.2 3,181,879 + PGA1_c30460 0.48 -0.5 3,181,887 - PGA1_c30460 0.48 -1.2 3,181,887 - PGA1_c30460 0.48 -1.7 3,181,887 - PGA1_c30460 0.48 -0.6 3,181,935 + PGA1_c30460 0.54 -1.0 3,181,966 + PGA1_c30460 0.58 -0.3 3,181,966 + PGA1_c30460 0.58 -0.9 3,181,966 + PGA1_c30460 0.58 -1.6 3,181,966 + PGA1_c30460 0.58 -0.2 3,181,974 - PGA1_c30460 0.59 -1.4 3,181,974 - PGA1_c30460 0.59 -0.9 3,181,974 - PGA1_c30460 0.59 -0.7 3,182,008 + PGA1_c30460 0.62 -0.9 3,182,047 + PGA1_c30460 0.67 -0.4 3,182,047 + PGA1_c30460 0.67 -0.5 3,182,050 - PGA1_c30460 0.67 -1.2 3,182,055 - PGA1_c30460 0.68 -1.4 3,182,055 - PGA1_c30460 0.68 -1.7 3,182,055 - PGA1_c30460 0.68 -1.2 3,182,064 + PGA1_c30460 0.69 -0.1 3,182,121 - PGA1_c30460 0.76 -0.6 3,182,131 + PGA1_c30460 0.77 -0.2 3,182,136 + PGA1_c30460 0.77 -1.9 3,182,219 - PGA1_c30460 0.87 -0.7 3,182,247 - -1.0 3,182,318 + -0.4 3,182,339 + +0.1 3,182,347 + +0.6 3,182,347 - +0.0 3,182,407 + -2.0 3,182,407 + -1.7 3,182,446 - PGA1_c30470 0.11 -2.4 3,182,487 + PGA1_c30470 0.15 -0.0 3,182,517 + PGA1_c30470 0.18 -2.8 3,182,576 + PGA1_c30470 0.24 -0.7 3,182,662 + PGA1_c30470 0.33 -0.8 3,182,662 + PGA1_c30470 0.33 -1.1 3,182,670 - PGA1_c30470 0.34 -2.0 3,182,773 + PGA1_c30470 0.45 -2.4 3,182,928 + PGA1_c30470 0.61 -1.6 3,183,085 + PGA1_c30470 0.77 -3.5 3,183,127 - PGA1_c30470 0.81 -1.7 3,183,150 - PGA1_c30470 0.84 -1.3 3,183,293 + -2.2 3,183,306 - -0.6 3,183,311 - -2.3 3,183,313 + -0.7 3,183,323 - -1.1
Or see this region's nucleotide sequence