Experiment: H2 N-free with 10mM L-Asparagine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02675 and MMP_RS02680 overlap by 14 nucleotides MMP_RS02680 and MMP_RS02685 are separated by 13 nucleotides MMP_RS02685 and MMP_RS02690 are separated by 586 nucleotides
MMP_RS02675: MMP_RS02675 - hypothetical protein, at 509,394 to 510,107
_RS02675
MMP_RS02680: MMP_RS02680 - ABC transporter permease, at 510,094 to 510,894
_RS02680
MMP_RS02685: MMP_RS02685 - molybdate ABC transporter substrate-binding protein, at 510,908 to 511,690
_RS02685
MMP_RS02690: MMP_RS02690 - FmdE family protein, at 512,277 to 512,852
_RS02690
Position (kb) 510
511
512 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 510.096 kb on + strand at 510.135 kb on - strand at 510.135 kb on - strand at 510.139 kb on - strand at 510.140 kb on + strand at 510.145 kb on - strand at 510.146 kb on + strand at 510.146 kb on + strand at 510.167 kb on + strand at 510.175 kb on - strand, within MMP_RS02680 at 510.448 kb on + strand, within MMP_RS02680 at 510.462 kb on - strand, within MMP_RS02680 at 510.527 kb on + strand, within MMP_RS02680 at 510.659 kb on - strand, within MMP_RS02680 at 510.706 kb on - strand, within MMP_RS02680 at 510.796 kb on - strand, within MMP_RS02680 at 510.821 kb on + strand at 510.941 kb on - strand at 511.021 kb on - strand, within MMP_RS02685 at 511.022 kb on + strand, within MMP_RS02685 at 511.039 kb on - strand, within MMP_RS02685 at 511.040 kb on + strand, within MMP_RS02685 at 511.045 kb on + strand, within MMP_RS02685 at 511.146 kb on + strand, within MMP_RS02685 at 511.403 kb on + strand, within MMP_RS02685 at 511.445 kb on - strand, within MMP_RS02685 at 511.446 kb on + strand, within MMP_RS02685 at 511.584 kb on - strand, within MMP_RS02685 at 511.585 kb on + strand, within MMP_RS02685 at 511.593 kb on + strand, within MMP_RS02685 at 511.596 kb on + strand, within MMP_RS02685 at 511.596 kb on + strand, within MMP_RS02685 at 511.781 kb on - strand at 511.782 kb on + strand at 511.782 kb on + strand at 511.785 kb on - strand at 511.989 kb on - strand at 512.139 kb on - strand at 512.140 kb on + strand at 512.191 kb on + strand at 512.194 kb on - strand at 512.194 kb on - strand at 512.195 kb on + strand at 512.250 kb on - strand at 512.251 kb on + strand at 512.262 kb on - strand at 512.263 kb on + strand at 512.275 kb on + strand at 512.275 kb on + strand at 512.275 kb on + strand at 512.282 kb on - strand at 512.344 kb on - strand, within MMP_RS02690 at 512.400 kb on + strand, within MMP_RS02690 at 512.611 kb on - strand, within MMP_RS02690 at 512.634 kb on - strand, within MMP_RS02690 at 512.675 kb on + strand, within MMP_RS02690 at 512.682 kb on + strand, within MMP_RS02690
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Asparagine remove 510,096 + -1.5 510,135 - -3.8 510,135 - -3.8 510,139 - -2.1 510,140 + -3.0 510,145 - -2.8 510,146 + -3.2 510,146 + -4.8 510,167 + -4.8 510,175 - MMP_RS02680 0.10 -1.8 510,448 + MMP_RS02680 0.44 -2.3 510,462 - MMP_RS02680 0.46 -2.5 510,527 + MMP_RS02680 0.54 -2.3 510,659 - MMP_RS02680 0.71 -1.5 510,706 - MMP_RS02680 0.76 -2.6 510,796 - MMP_RS02680 0.88 -2.9 510,821 + -2.8 510,941 - -2.4 511,021 - MMP_RS02685 0.14 -3.1 511,022 + MMP_RS02685 0.15 -3.5 511,039 - MMP_RS02685 0.17 -2.8 511,040 + MMP_RS02685 0.17 -3.2 511,045 + MMP_RS02685 0.17 -3.1 511,146 + MMP_RS02685 0.30 -2.3 511,403 + MMP_RS02685 0.63 -3.7 511,445 - MMP_RS02685 0.69 -3.3 511,446 + MMP_RS02685 0.69 -3.0 511,584 - MMP_RS02685 0.86 -3.2 511,585 + MMP_RS02685 0.86 -3.6 511,593 + MMP_RS02685 0.87 -3.0 511,596 + MMP_RS02685 0.88 -2.8 511,596 + MMP_RS02685 0.88 -2.3 511,781 - -3.0 511,782 + -2.7 511,782 + -1.3 511,785 - -2.2 511,989 - +0.5 512,139 - +0.2 512,140 + +1.4 512,191 + -0.2 512,194 - +0.5 512,194 - +0.4 512,195 + +0.0 512,250 - +0.9 512,251 + +0.2 512,262 - +0.6 512,263 + +0.1 512,275 + -0.2 512,275 + +0.6 512,275 + -0.7 512,282 - +1.0 512,344 - MMP_RS02690 0.12 +0.4 512,400 + MMP_RS02690 0.21 +0.6 512,611 - MMP_RS02690 0.58 +0.3 512,634 - MMP_RS02690 0.62 -0.0 512,675 + MMP_RS02690 0.69 +0.1 512,682 + MMP_RS02690 0.70 -0.1
Or see this region's nucleotide sequence