Experiment: H2 N-free with 10mM L-Asparagine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02660 and MMP_RS02665 are separated by 411 nucleotides MMP_RS02665 and MMP_RS02670 are separated by 55 nucleotides MMP_RS02670 and MMP_RS02675 are separated by 16 nucleotides MMP_RS02675 and MMP_RS02680 overlap by 14 nucleotides
MMP_RS02660: MMP_RS02660 - hypothetical protein, at 506,485 to 507,573
_RS02660
MMP_RS02665: MMP_RS02665 - hypothetical protein, at 507,985 to 508,290
_RS02665
MMP_RS02670: MMP_RS02670 - ATP-binding cassette domain-containing protein, at 508,346 to 509,377
_RS02670
MMP_RS02675: MMP_RS02675 - hypothetical protein, at 509,394 to 510,107
_RS02675
MMP_RS02680: MMP_RS02680 - ABC transporter permease, at 510,094 to 510,894
_RS02680
Position (kb) 508
509
510 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 507.356 kb on - strand, within MMP_RS02660 at 507.357 kb on + strand, within MMP_RS02660 at 507.360 kb on - strand, within MMP_RS02660 at 507.360 kb on - strand, within MMP_RS02660 at 507.360 kb on - strand, within MMP_RS02660 at 507.392 kb on + strand, within MMP_RS02660 at 507.407 kb on + strand, within MMP_RS02660 at 507.412 kb on - strand, within MMP_RS02660 at 507.464 kb on + strand, within MMP_RS02660 at 507.556 kb on + strand at 507.603 kb on - strand at 507.604 kb on + strand at 507.626 kb on + strand at 507.696 kb on + strand at 507.733 kb on + strand at 507.753 kb on - strand at 507.761 kb on - strand at 507.762 kb on + strand at 507.764 kb on + strand at 507.812 kb on + strand at 507.826 kb on + strand at 507.878 kb on - strand at 508.288 kb on - strand at 508.293 kb on + strand at 508.304 kb on + strand at 508.375 kb on + strand at 508.448 kb on - strand at 508.448 kb on - strand at 508.449 kb on + strand at 508.449 kb on + strand at 508.523 kb on - strand, within MMP_RS02670 at 508.558 kb on - strand, within MMP_RS02670 at 508.558 kb on - strand, within MMP_RS02670 at 508.645 kb on + strand, within MMP_RS02670 at 508.730 kb on - strand, within MMP_RS02670 at 508.754 kb on + strand, within MMP_RS02670 at 508.761 kb on + strand, within MMP_RS02670 at 508.761 kb on + strand, within MMP_RS02670 at 508.778 kb on - strand, within MMP_RS02670 at 508.779 kb on + strand, within MMP_RS02670 at 508.832 kb on - strand, within MMP_RS02670 at 508.833 kb on + strand, within MMP_RS02670 at 508.835 kb on + strand, within MMP_RS02670 at 508.869 kb on + strand, within MMP_RS02670 at 508.875 kb on - strand, within MMP_RS02670 at 508.895 kb on - strand, within MMP_RS02670 at 508.896 kb on + strand, within MMP_RS02670 at 508.949 kb on - strand, within MMP_RS02670 at 509.047 kb on - strand, within MMP_RS02670 at 509.048 kb on + strand, within MMP_RS02670 at 509.296 kb on + strand at 509.328 kb on - strand at 509.328 kb on - strand at 509.329 kb on + strand at 509.369 kb on - strand at 509.369 kb on - strand at 509.378 kb on - strand at 509.379 kb on + strand at 509.383 kb on + strand at 509.495 kb on + strand, within MMP_RS02675 at 509.586 kb on + strand, within MMP_RS02675 at 509.868 kb on + strand, within MMP_RS02675 at 510.096 kb on + strand at 510.135 kb on - strand at 510.135 kb on - strand at 510.139 kb on - strand at 510.140 kb on + strand at 510.145 kb on - strand at 510.146 kb on + strand at 510.146 kb on + strand at 510.167 kb on + strand at 510.175 kb on - strand, within MMP_RS02680
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Asparagine remove 507,356 - MMP_RS02660 0.80 +0.0 507,357 + MMP_RS02660 0.80 +0.0 507,360 - MMP_RS02660 0.80 -1.1 507,360 - MMP_RS02660 0.80 +0.0 507,360 - MMP_RS02660 0.80 +1.2 507,392 + MMP_RS02660 0.83 -0.6 507,407 + MMP_RS02660 0.85 -0.8 507,412 - MMP_RS02660 0.85 +0.8 507,464 + MMP_RS02660 0.90 +0.5 507,556 + +0.0 507,603 - +0.6 507,604 + -1.0 507,626 + +0.3 507,696 + +0.4 507,733 + -0.3 507,753 - +0.1 507,761 - -0.4 507,762 + +0.7 507,764 + +0.1 507,812 + +0.3 507,826 + +0.0 507,878 - +0.7 508,288 - -0.2 508,293 + -0.3 508,304 + +0.1 508,375 + -3.5 508,448 - -2.5 508,448 - -2.6 508,449 + -2.5 508,449 + -3.1 508,523 - MMP_RS02670 0.17 -2.8 508,558 - MMP_RS02670 0.21 -2.2 508,558 - MMP_RS02670 0.21 -1.8 508,645 + MMP_RS02670 0.29 -3.7 508,730 - MMP_RS02670 0.37 -2.5 508,754 + MMP_RS02670 0.40 -1.5 508,761 + MMP_RS02670 0.40 -3.3 508,761 + MMP_RS02670 0.40 -3.8 508,778 - MMP_RS02670 0.42 -2.3 508,779 + MMP_RS02670 0.42 -2.8 508,832 - MMP_RS02670 0.47 -3.5 508,833 + MMP_RS02670 0.47 -3.5 508,835 + MMP_RS02670 0.47 -2.6 508,869 + MMP_RS02670 0.51 -4.5 508,875 - MMP_RS02670 0.51 -2.0 508,895 - MMP_RS02670 0.53 -2.9 508,896 + MMP_RS02670 0.53 -3.8 508,949 - MMP_RS02670 0.58 -1.2 509,047 - MMP_RS02670 0.68 -3.4 509,048 + MMP_RS02670 0.68 -3.3 509,296 + -3.0 509,328 - -1.6 509,328 - -3.8 509,329 + -2.9 509,369 - -2.9 509,369 - -2.1 509,378 - -2.6 509,379 + -2.4 509,383 + -2.0 509,495 + MMP_RS02675 0.14 -3.6 509,586 + MMP_RS02675 0.27 -3.5 509,868 + MMP_RS02675 0.66 -3.0 510,096 + -1.5 510,135 - -3.8 510,135 - -3.8 510,139 - -2.1 510,140 + -3.0 510,145 - -2.8 510,146 + -3.2 510,146 + -4.8 510,167 + -4.8 510,175 - MMP_RS02680 0.10 -1.8
Or see this region's nucleotide sequence