Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS27435

Experiment: Fusidic 1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS27430 and BWI76_RS27435 are separated by 4 nucleotidesBWI76_RS27435 and BWI76_RS27440 are separated by 2 nucleotides BWI76_RS27430: BWI76_RS27430 - (p)ppGpp synthetase, at 5,689,947 to 5,692,067 _RS27430 BWI76_RS27435: BWI76_RS27435 - tRNA (guanosine(18)-2'-O)-methyltransferase TrmH, at 5,692,072 to 5,692,761 _RS27435 BWI76_RS27440: BWI76_RS27440 - DNA helicase RecG, at 5,692,764 to 5,694,845 _RS27440 Position (kb) 5692 5693Strain fitness (log2 ratio) -3 -2 -1 0 1at 5691.447 kb on - strand, within BWI76_RS27430at 5691.721 kb on - strand, within BWI76_RS27430at 5691.896 kb on + strandat 5692.004 kb on - strandat 5692.139 kb on + strandat 5692.287 kb on + strand, within BWI76_RS27435at 5692.295 kb on - strand, within BWI76_RS27435at 5692.295 kb on - strand, within BWI76_RS27435at 5692.333 kb on + strand, within BWI76_RS27435at 5692.333 kb on + strand, within BWI76_RS27435at 5692.420 kb on - strand, within BWI76_RS27435at 5692.471 kb on + strand, within BWI76_RS27435at 5692.545 kb on + strand, within BWI76_RS27435at 5692.553 kb on - strand, within BWI76_RS27435at 5692.605 kb on + strand, within BWI76_RS27435at 5692.678 kb on + strand, within BWI76_RS27435at 5692.868 kb on - strandat 5693.250 kb on - strand, within BWI76_RS27440at 5693.383 kb on + strand, within BWI76_RS27440at 5693.391 kb on - strand, within BWI76_RS27440at 5693.506 kb on + strand, within BWI76_RS27440

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Per-strain Table

Position Strand Gene LocusTag Fraction Fusidic 1 mg/ml
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5,691,447 - BWI76_RS27430 0.71 +1.4
5,691,721 - BWI76_RS27430 0.84 -0.5
5,691,896 + +1.3
5,692,004 - -0.8
5,692,139 + +0.1
5,692,287 + BWI76_RS27435 0.31 -0.1
5,692,295 - BWI76_RS27435 0.32 -3.2
5,692,295 - BWI76_RS27435 0.32 +0.3
5,692,333 + BWI76_RS27435 0.38 -1.1
5,692,333 + BWI76_RS27435 0.38 -1.7
5,692,420 - BWI76_RS27435 0.50 -3.0
5,692,471 + BWI76_RS27435 0.58 -1.3
5,692,545 + BWI76_RS27435 0.69 -0.9
5,692,553 - BWI76_RS27435 0.70 -2.5
5,692,605 + BWI76_RS27435 0.77 -0.7
5,692,678 + BWI76_RS27435 0.88 -0.3
5,692,868 - -2.3
5,693,250 - BWI76_RS27440 0.23 -1.6
5,693,383 + BWI76_RS27440 0.30 -0.6
5,693,391 - BWI76_RS27440 0.30 -1.6
5,693,506 + BWI76_RS27440 0.36 -1.6

Or see this region's nucleotide sequence