Experiment: Fusidic 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19860 and BWI76_RS19865 are separated by 338 nucleotides BWI76_RS19865 and BWI76_RS19870 are separated by 42 nucleotides BWI76_RS19870 and BWI76_RS19875 are separated by 279 nucleotides
BWI76_RS19860: BWI76_RS19860 - hypothetical protein, at 4,152,369 to 4,152,791
_RS19860
BWI76_RS19865: BWI76_RS19865 - ecotin, at 4,153,130 to 4,153,621
_RS19865
BWI76_RS19870: BWI76_RS19870 - malate:quinone oxidoreductase, at 4,153,664 to 4,155,298
_RS19870
BWI76_RS19875: BWI76_RS19875 - sulfate transporter, at 4,155,578 to 4,156,825
_RS19875
Position (kb)
4153
4154
4155
4156 Strain fitness (log2 ratio)
-1
0
1
2 at 4153.005 kb on + strand at 4153.011 kb on - strand at 4153.060 kb on + strand at 4153.062 kb on + strand at 4153.163 kb on - strand at 4153.249 kb on - strand, within BWI76_RS19865 at 4153.396 kb on - strand, within BWI76_RS19865 at 4153.396 kb on - strand, within BWI76_RS19865 at 4153.607 kb on + strand at 4154.199 kb on + strand, within BWI76_RS19870 at 4154.541 kb on - strand, within BWI76_RS19870 at 4154.588 kb on + strand at 4154.888 kb on + strand, within BWI76_RS19870 at 4155.036 kb on + strand, within BWI76_RS19870 at 4155.053 kb on - strand, within BWI76_RS19870 at 4155.273 kb on - strand at 4155.650 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 1 mg/ml remove 4,153,005 + -0.3 4,153,011 - +0.9 4,153,060 + -0.8 4,153,062 + +0.1 4,153,163 - -0.9 4,153,249 - BWI76_RS19865 0.24 -0.3 4,153,396 - BWI76_RS19865 0.54 +1.3 4,153,396 - BWI76_RS19865 0.54 +0.3 4,153,607 + -0.4 4,154,199 + BWI76_RS19870 0.33 +0.2 4,154,541 - BWI76_RS19870 0.54 +0.1 4,154,588 + +2.4 4,154,888 + BWI76_RS19870 0.75 +0.1 4,155,036 + BWI76_RS19870 0.84 +2.2 4,155,053 - BWI76_RS19870 0.85 +1.4 4,155,273 - +0.1 4,155,650 + +0.3
Or see this region's nucleotide sequence