Experiment: Fusidic 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19770 and BWI76_RS19775 are separated by 190 nucleotides BWI76_RS19775 and BWI76_RS19780 are separated by 70 nucleotides
BWI76_RS19770: BWI76_RS19770 - bifunctional murein DD-endopeptidase/murein LD-carboxypeptidase, at 4,133,295 to 4,133,864
_RS19770
BWI76_RS19775: BWI76_RS19775 - phage resistance protein, at 4,134,055 to 4,135,617
_RS19775
BWI76_RS19780: BWI76_RS19780 - antibiotic ABC transporter substrate-binding protein, at 4,135,688 to 4,137,493
_RS19780
Position (kb) 4134
4135
4136 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4133.124 kb on - strand at 4133.124 kb on - strand at 4133.124 kb on - strand at 4133.281 kb on + strand at 4133.289 kb on - strand at 4133.289 kb on - strand at 4133.358 kb on + strand, within BWI76_RS19770 at 4133.366 kb on - strand, within BWI76_RS19770 at 4133.847 kb on + strand at 4134.051 kb on + strand at 4134.059 kb on - strand at 4134.202 kb on + strand at 4134.202 kb on + strand at 4134.202 kb on + strand at 4134.911 kb on + strand, within BWI76_RS19775 at 4134.919 kb on - strand, within BWI76_RS19775 at 4135.463 kb on - strand at 4135.637 kb on + strand at 4135.704 kb on + strand at 4135.757 kb on - strand at 4135.811 kb on - strand at 4135.811 kb on - strand at 4135.859 kb on - strand at 4135.932 kb on + strand, within BWI76_RS19780 at 4135.954 kb on - strand, within BWI76_RS19780 at 4136.348 kb on + strand, within BWI76_RS19780 at 4136.474 kb on + strand, within BWI76_RS19780
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 1 mg/ml remove 4,133,124 - -1.1 4,133,124 - +1.2 4,133,124 - -0.5 4,133,281 + -0.2 4,133,289 - -1.5 4,133,289 - -0.5 4,133,358 + BWI76_RS19770 0.11 -1.3 4,133,366 - BWI76_RS19770 0.12 -0.2 4,133,847 + -0.1 4,134,051 + -1.8 4,134,059 - +0.3 4,134,202 + +1.1 4,134,202 + +0.1 4,134,202 + +0.1 4,134,911 + BWI76_RS19775 0.55 +3.1 4,134,919 - BWI76_RS19775 0.55 +0.4 4,135,463 - +0.9 4,135,637 + -0.1 4,135,704 + -0.5 4,135,757 - +0.2 4,135,811 - -1.1 4,135,811 - -0.3 4,135,859 - -0.2 4,135,932 + BWI76_RS19780 0.14 -0.3 4,135,954 - BWI76_RS19780 0.15 +3.1 4,136,348 + BWI76_RS19780 0.37 -0.3 4,136,474 + BWI76_RS19780 0.44 -0.1
Or see this region's nucleotide sequence