Experiment: Fusidic 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19570 and BWI76_RS19575 are separated by 8 nucleotides BWI76_RS19575 and BWI76_RS19580 are separated by 8 nucleotides BWI76_RS19580 and BWI76_RS19585 are separated by 24 nucleotides
BWI76_RS19570: BWI76_RS19570 - 3-hydroxybenzoate 6-hydroxylase, at 4,091,793 to 4,092,986
_RS19570
BWI76_RS19575: BWI76_RS19575 - maleylacetoacetate isomerase, at 4,092,995 to 4,093,639
_RS19575
BWI76_RS19580: BWI76_RS19580 - FAA-hydrolase, at 4,093,648 to 4,094,346
_RS19580
BWI76_RS19585: BWI76_RS19585 - gentisate 1,2-dioxygenase, at 4,094,371 to 4,095,408
_RS19585
Position (kb)
4092
4093
4094 Strain fitness (log2 ratio)
-1
0
1 at 4092.031 kb on + strand, within BWI76_RS19570 at 4092.062 kb on - strand, within BWI76_RS19570 at 4092.084 kb on + strand, within BWI76_RS19570 at 4092.087 kb on - strand, within BWI76_RS19570 at 4092.705 kb on + strand, within BWI76_RS19570 at 4092.977 kb on + strand at 4093.080 kb on - strand, within BWI76_RS19575 at 4093.158 kb on - strand, within BWI76_RS19575 at 4093.886 kb on - strand, within BWI76_RS19580 at 4093.996 kb on + strand, within BWI76_RS19580 at 4094.384 kb on + strand at 4094.384 kb on + strand at 4094.553 kb on - strand, within BWI76_RS19585
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 1 mg/ml remove 4,092,031 + BWI76_RS19570 0.20 -0.2 4,092,062 - BWI76_RS19570 0.23 -0.7 4,092,084 + BWI76_RS19570 0.24 -0.5 4,092,087 - BWI76_RS19570 0.25 +0.3 4,092,705 + BWI76_RS19570 0.76 +0.4 4,092,977 + -0.5 4,093,080 - BWI76_RS19575 0.13 -1.3 4,093,158 - BWI76_RS19575 0.25 -0.2 4,093,886 - BWI76_RS19580 0.34 +0.7 4,093,996 + BWI76_RS19580 0.50 -0.2 4,094,384 + +0.3 4,094,384 + -0.1 4,094,553 - BWI76_RS19585 0.18 -0.3
Or see this region's nucleotide sequence