Experiment: Fusidic 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16120 and BWI76_RS16125 overlap by 11 nucleotides BWI76_RS16125 and BWI76_RS16130 overlap by 11 nucleotides BWI76_RS16130 and BWI76_RS16135 are separated by 9 nucleotides
BWI76_RS16120: BWI76_RS16120 - 4-carboxymuconolactone decarboxylase, at 3,345,258 to 3,345,641
_RS16120
BWI76_RS16125: BWI76_RS16125 - 3-oxoadipate enol-lactonase, at 3,345,631 to 3,346,398
_RS16125
BWI76_RS16130: BWI76_RS16130 - 3-carboxy-cis,cis-muconate cycloisomerase, at 3,346,388 to 3,347,740
_RS16130
BWI76_RS16135: BWI76_RS16135 - acetyl-CoA acetyltransferase, at 3,347,750 to 3,348,952
_RS16135
Position (kb) 3346
3347
3348 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 3345.392 kb on - strand, within BWI76_RS16120 at 3345.440 kb on + strand, within BWI76_RS16120 at 3345.585 kb on + strand, within BWI76_RS16120 at 3345.593 kb on - strand, within BWI76_RS16120 at 3345.654 kb on + strand at 3345.903 kb on + strand, within BWI76_RS16125 at 3346.488 kb on - strand at 3346.494 kb on + strand at 3346.851 kb on + strand, within BWI76_RS16130 at 3347.026 kb on - strand, within BWI76_RS16130 at 3347.070 kb on - strand, within BWI76_RS16130 at 3347.259 kb on - strand, within BWI76_RS16130 at 3347.785 kb on - strand at 3347.918 kb on + strand, within BWI76_RS16135 at 3347.941 kb on + strand, within BWI76_RS16135 at 3347.949 kb on - strand, within BWI76_RS16135 at 3348.604 kb on - strand, within BWI76_RS16135
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 1 mg/ml remove 3,345,392 - BWI76_RS16120 0.35 +0.3 3,345,440 + BWI76_RS16120 0.47 -2.9 3,345,585 + BWI76_RS16120 0.85 +0.4 3,345,593 - BWI76_RS16120 0.87 -0.6 3,345,654 + +0.5 3,345,903 + BWI76_RS16125 0.35 +2.0 3,346,488 - -1.0 3,346,494 + +0.4 3,346,851 + BWI76_RS16130 0.34 -0.2 3,347,026 - BWI76_RS16130 0.47 +1.8 3,347,070 - BWI76_RS16130 0.50 +0.4 3,347,259 - BWI76_RS16130 0.64 +0.2 3,347,785 - +0.4 3,347,918 + BWI76_RS16135 0.14 +0.1 3,347,941 + BWI76_RS16135 0.16 +0.0 3,347,949 - BWI76_RS16135 0.17 +0.1 3,348,604 - BWI76_RS16135 0.71 +0.8
Or see this region's nucleotide sequence