Experiment: Fusidic 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15120 and BWI76_RS15125 overlap by 7 nucleotides BWI76_RS15125 and BWI76_RS15130 overlap by 4 nucleotides BWI76_RS15130 and BWI76_RS15135 are separated by 300 nucleotides BWI76_RS15135 and BWI76_RS15140 are separated by 35 nucleotides
BWI76_RS15120: BWI76_RS15120 - hypothetical protein, at 3,170,405 to 3,170,623
_RS15120
BWI76_RS15125: BWI76_RS15125 - acetyltransferase, at 3,170,617 to 3,171,195
_RS15125
BWI76_RS15130: BWI76_RS15130 - hypothetical protein, at 3,171,192 to 3,171,638
_RS15130
BWI76_RS15135: BWI76_RS15135 - hypothetical protein, at 3,171,939 to 3,172,229
_RS15135
BWI76_RS15140: BWI76_RS15140 - CusA/CzcA family heavy metal efflux RND transporter, at 3,172,265 to 3,175,378
_RS15140
Position (kb)
3171
3172 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3170.213 kb on + strand at 3170.300 kb on + strand at 3170.346 kb on + strand at 3170.402 kb on + strand at 3170.402 kb on + strand at 3170.402 kb on + strand at 3170.410 kb on - strand at 3170.410 kb on - strand at 3170.410 kb on - strand at 3170.410 kb on - strand at 3170.410 kb on - strand at 3170.418 kb on - strand at 3170.422 kb on + strand at 3170.422 kb on + strand at 3170.423 kb on - strand at 3170.430 kb on - strand, within BWI76_RS15120 at 3170.439 kb on - strand, within BWI76_RS15120 at 3170.445 kb on - strand at 3170.496 kb on - strand, within BWI76_RS15120 at 3170.511 kb on + strand, within BWI76_RS15120 at 3170.519 kb on - strand at 3170.548 kb on - strand, within BWI76_RS15120 at 3170.625 kb on + strand at 3170.633 kb on - strand at 3170.655 kb on - strand at 3170.707 kb on - strand, within BWI76_RS15125 at 3170.814 kb on - strand, within BWI76_RS15125 at 3170.822 kb on - strand, within BWI76_RS15125 at 3170.880 kb on + strand, within BWI76_RS15125 at 3170.991 kb on + strand, within BWI76_RS15125 at 3171.183 kb on - strand at 3171.359 kb on + strand, within BWI76_RS15130 at 3171.413 kb on - strand, within BWI76_RS15130 at 3171.745 kb on - strand at 3171.904 kb on - strand at 3172.060 kb on - strand, within BWI76_RS15135 at 3172.090 kb on - strand, within BWI76_RS15135 at 3172.159 kb on + strand, within BWI76_RS15135 at 3172.167 kb on - strand, within BWI76_RS15135 at 3172.346 kb on + strand at 3172.542 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 1 mg/ml remove 3,170,213 + +1.0 3,170,300 + -0.1 3,170,346 + -0.5 3,170,402 + +0.5 3,170,402 + -1.1 3,170,402 + -0.3 3,170,410 - -1.0 3,170,410 - +0.5 3,170,410 - +0.0 3,170,410 - -1.5 3,170,410 - +0.3 3,170,418 - +0.4 3,170,422 + +0.5 3,170,422 + +0.3 3,170,423 - +1.3 3,170,430 - BWI76_RS15120 0.11 -0.5 3,170,439 - BWI76_RS15120 0.16 +0.7 3,170,445 - +2.0 3,170,496 - BWI76_RS15120 0.42 -1.4 3,170,511 + BWI76_RS15120 0.48 -1.9 3,170,519 - +3.6 3,170,548 - BWI76_RS15120 0.65 -0.1 3,170,625 + +0.7 3,170,633 - +0.2 3,170,655 - +0.7 3,170,707 - BWI76_RS15125 0.16 +0.7 3,170,814 - BWI76_RS15125 0.34 -1.3 3,170,822 - BWI76_RS15125 0.35 +0.2 3,170,880 + BWI76_RS15125 0.45 -0.5 3,170,991 + BWI76_RS15125 0.65 +1.0 3,171,183 - -1.2 3,171,359 + BWI76_RS15130 0.37 -1.5 3,171,413 - BWI76_RS15130 0.49 -0.0 3,171,745 - +0.2 3,171,904 - -1.0 3,172,060 - BWI76_RS15135 0.42 +0.7 3,172,090 - BWI76_RS15135 0.52 +0.8 3,172,159 + BWI76_RS15135 0.76 -0.3 3,172,167 - BWI76_RS15135 0.78 +0.1 3,172,346 + -0.9 3,172,542 - -0.1
Or see this region's nucleotide sequence