Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS06475

Experiment: Fusidic 1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS06465 and BWI76_RS06470 are separated by 45 nucleotidesBWI76_RS06470 and BWI76_RS06475 are separated by 295 nucleotidesBWI76_RS06475 and BWI76_RS06480 are separated by 11 nucleotidesBWI76_RS06480 and BWI76_RS06485 overlap by 1 nucleotidesBWI76_RS06485 and BWI76_RS06490 overlap by 11 nucleotides BWI76_RS06465: BWI76_RS06465 - IclR family transcriptional regulator, at 1,359,105 to 1,359,863 _RS06465 BWI76_RS06470: BWI76_RS06470 - hypothetical protein, at 1,359,909 to 1,360,241 _RS06470 BWI76_RS06475: BWI76_RS06475 - protoheme IX farnesyltransferase, at 1,360,537 to 1,361,424 _RS06475 BWI76_RS06480: BWI76_RS06480 - cytochrome o ubiquinol oxidase subunit IV, at 1,361,436 to 1,361,765 _RS06480 BWI76_RS06485: BWI76_RS06485 - cytochrome o ubiquinol oxidase subunit III, at 1,361,765 to 1,362,376 _RS06485 BWI76_RS06490: BWI76_RS06490 - cytochrome ubiquinol oxidase subunit I, at 1,362,366 to 1,364,357 _RS06490 Position (kb) 1360 1361 1362Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1360.033 kb on - strand, within BWI76_RS06470at 1360.147 kb on + strand, within BWI76_RS06470at 1360.190 kb on + strand, within BWI76_RS06470at 1360.277 kb on + strandat 1360.277 kb on + strandat 1360.277 kb on + strandat 1360.287 kb on - strandat 1360.287 kb on - strandat 1360.305 kb on - strandat 1360.305 kb on - strandat 1360.313 kb on - strandat 1360.546 kb on - strandat 1360.575 kb on + strandat 1360.842 kb on + strand, within BWI76_RS06475at 1361.081 kb on - strand, within BWI76_RS06475at 1361.591 kb on - strand, within BWI76_RS06480at 1361.856 kb on - strand, within BWI76_RS06485at 1361.946 kb on + strand, within BWI76_RS06485

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Per-strain Table

Position Strand Gene LocusTag Fraction Fusidic 1 mg/ml
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1,360,033 - BWI76_RS06470 0.37 +0.3
1,360,147 + BWI76_RS06470 0.71 +1.0
1,360,190 + BWI76_RS06470 0.84 +0.6
1,360,277 + -0.5
1,360,277 + -2.2
1,360,277 + +0.8
1,360,287 - +0.7
1,360,287 - +0.5
1,360,305 - -0.3
1,360,305 - -0.2
1,360,313 - +0.0
1,360,546 - +0.1
1,360,575 + -0.4
1,360,842 + BWI76_RS06475 0.34 +2.8
1,361,081 - BWI76_RS06475 0.61 -0.9
1,361,591 - BWI76_RS06480 0.47 -0.3
1,361,856 - BWI76_RS06485 0.15 +0.1
1,361,946 + BWI76_RS06485 0.30 +0.1

Or see this region's nucleotide sequence