Experiment: Fusidic 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01185 and BWI76_RS01190 are separated by 80 nucleotides BWI76_RS01190 and BWI76_RS01195 are separated by 138 nucleotides BWI76_RS01195 and BWI76_RS01200 are separated by 25 nucleotides
BWI76_RS01185: BWI76_RS01185 - adenylate cyclase, at 242,042 to 244,591
_RS01185
BWI76_RS01190: BWI76_RS01190 - iron donor protein CyaY, at 244,672 to 244,992
_RS01190
BWI76_RS01195: BWI76_RS01195 - membrane protein, at 245,131 to 245,334
_RS01195
BWI76_RS01200: BWI76_RS01200 - diaminopimelate epimerase, at 245,360 to 246,184
_RS01200
Position (kb) 244
245 Strain fitness (log2 ratio) -1
0
1 at 243.956 kb on - strand, within BWI76_RS01185 at 243.956 kb on - strand, within BWI76_RS01185 at 244.498 kb on + strand at 244.555 kb on - strand at 244.608 kb on + strand at 244.876 kb on - strand, within BWI76_RS01190 at 244.920 kb on - strand, within BWI76_RS01190 at 245.186 kb on + strand, within BWI76_RS01195 at 245.194 kb on - strand, within BWI76_RS01195 at 245.337 kb on + strand at 245.809 kb on - strand, within BWI76_RS01200 at 245.809 kb on - strand, within BWI76_RS01200 at 245.838 kb on + strand, within BWI76_RS01200 at 245.838 kb on + strand, within BWI76_RS01200
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 1 mg/ml remove 243,956 - BWI76_RS01185 0.75 +0.5 243,956 - BWI76_RS01185 0.75 -0.8 244,498 + -0.2 244,555 - +0.2 244,608 + -0.3 244,876 - BWI76_RS01190 0.64 +0.7 244,920 - BWI76_RS01190 0.77 +0.7 245,186 + BWI76_RS01195 0.27 -0.4 245,194 - BWI76_RS01195 0.31 +0.6 245,337 + +0.6 245,809 - BWI76_RS01200 0.54 -0.0 245,809 - BWI76_RS01200 0.54 -0.5 245,838 + BWI76_RS01200 0.58 -1.5 245,838 + BWI76_RS01200 0.58 -1.1
Or see this region's nucleotide sequence