Experiment: MoLS4 with L-Threonine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rrl and tRNA-Ala are separated by 152 nucleotides tRNA-Ala and tRNA-Ile are separated by 22 nucleotides tRNA-Ile and rrsE are separated by 73 nucleotides rrsE and DVU3106 are separated by 511 nucleotides
Dv23SE: rrl - 23S ribosomal RNA (VIMSS-AUTO), at 3,247,287 to 3,250,207
rrl
DVU_tRNA-Ala-8: tRNA-Ala - tRNA with anticodon TGC for Ala (VIMSS-AUTO), at 3,250,360 to 3,250,435
tRNA-Ala
DVU_tRNA-Ile-5: tRNA-Ile - tRNA with anticodon GAT for Ile (VIMSS-AUTO), at 3,250,458 to 3,250,534
tRNA-Ile
Dv16SE: rrsE - 16S ribosomal RNA (RefSeq), at 3,250,608 to 3,252,148
rrsE
DVU3106: DVU3106 - GGDEF domain protein (TIGR), at 3,252,660 to 3,254,552
DVU3106
Position (kb)
3250
3251
3252
3253 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3252.510 kb on - strand at 3252.589 kb on + strand at 3252.628 kb on + strand at 3252.628 kb on + strand at 3252.656 kb on + strand at 3252.682 kb on + strand at 3252.745 kb on + strand at 3252.747 kb on - strand at 3252.747 kb on - strand at 3252.753 kb on - strand at 3252.785 kb on - strand at 3252.787 kb on + strand at 3252.792 kb on + strand at 3252.800 kb on - strand at 3252.828 kb on - strand at 3252.907 kb on + strand, within DVU3106 at 3252.919 kb on + strand, within DVU3106 at 3252.929 kb on + strand, within DVU3106 at 3253.047 kb on + strand, within DVU3106 at 3253.089 kb on + strand, within DVU3106
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Threonine (N) remove 3,252,510 - -0.3 3,252,589 + -0.1 3,252,628 + +2.2 3,252,628 + -0.6 3,252,656 + -0.9 3,252,682 + +0.8 3,252,745 + -0.5 3,252,747 - +0.9 3,252,747 - +3.3 3,252,753 - -0.3 3,252,785 - -1.2 3,252,787 + -0.1 3,252,792 + -0.5 3,252,800 - +2.1 3,252,828 - -0.3 3,252,907 + DVU3106 0.13 -1.2 3,252,919 + DVU3106 0.14 -2.5 3,252,929 + DVU3106 0.14 -0.1 3,253,047 + DVU3106 0.20 +3.4 3,253,089 + DVU3106 0.23 -1.5
Or see this region's nucleotide sequence